Known wider contexts of bla_NDM-1. Extents and orientations of selected resistance and other genes are shown by arrows labeled with the gene name. Different types of horizontal lines indicate plasmid backbone or the A. baumannii chromosome. IS are shown as boxes labeled with the name/number of the IS or shown in the key with the pointed end corresponding to the IR_R (or oriIS for ISCR elements). IS flanked by direct repeats (DRs) have generally been omitted, with vertical arrows indicating their locations. Tall bars represent Tn3-like 38-bp IR or IRi of class 1 In/Tn, as indicated. Boxes containing letters represent different cassette arrays (see key). DRs are shown by filled circles of the same color on short stalks, and nonmatching sequences of expected DR length are given. The vertical arrow on the AJ627643 diagram indicates the site of insertion of Tn125 in pNDM-BJ01. The dotted lines below the pMR0211 structure indicate possible inversion events due to recombination between fragments of class 1 integrons, and 6.564 kb of the IncA/C backbone is missing at the right-hand end compared with pNDM-1_Dok01. The pNDM10505 structure could be derived from the pNDM102337 structure by insertion of ISKpn14 in ISAba125, the pNDM10469 structure by recombination between these two ISKpn14 to delete the intervening segment (dotted line), and the pNDM-HK structure by switching of the cassette array. In these four plasmids, 3.473 kb of the IncA/C backbone is missing at the right-hand end compared with pNDM-1_Dok01. The BamHI site shown on the pNDM-HK structure marks the end of the bla_DHA-1 fragment in the K. pneumoniae 05-506/pKpANDM-1 (untyped plasmid; GenBank accession number FN396876) context (). Several other partial bla_NDM-1 contexts are illustrated in Fig. 2 of reference and Fig. 3 of reference , but in both of these figures ISAba125 is shown in the wrong orientation for E. coli 271 and potentially in other structures (the sequences are not available) and the ISAba125Δ-bla_NDM-1-ble portion of the K. pneumoniae 05-506 structure is also shown in the wrong orientation; i.e., IS26 should truncate ISAba125. Diagrams were drawn from the following GenBank accession numbers: TnaphA6, JF343537; Tn125, HQ857107; pNDM-BJ01, JQ001791; pNDM-BJ02, JQ060896; pAB_D499, AGFH01000030; pNDM-1_Dok01, AP012208; pRMH760, AY123253; pMR0211, JN687470; pNDM102337, JF714412; pNDM10505, JF503991; pNDM10469, JN861072; pNDM-KN, JN157804; pCTX-M3, AF550415; pNDM-HK, HQ451074; E. coli DVR22, JF922606; p271A, JF785549; pCK41, HQ332785.