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J Gen Virol. 2012 Dec;93(Pt 12):2595-2600. doi: 10.1099/vir.0.045302-0. Epub 2012 Aug 29.

Identification of a novel B-cell epitope of Hantaan virus glycoprotein recognized by neutralizing 3D8 monoclonal antibody.

Author information

1
Department of Immunology, the Fourth Military Medical University, 17 Changle West Road, Xi'an, Shaanxi 710032, PR China.
2
Department of Microbiology, the Fourth Military Medical University, 17 Changle West Road, Xi'an, Shaanxi 710032, PR China.

Abstract

Hantaan virus (HTNV), a member of the family Bunyaviridae, is a major agent causing haemorrhagic fever with renal syndrome, a high-mortality-rate disease threatening approximately 150 000 people around the world yearly. The 3D8 mAb displays a neutralizing activity to HTNV infection. In this study, the B-cell epitopes of HTNV glycoproteins (GPs) were finely mapped by peptide scanning. A new B-cell epitope (882)GFLCPEFPGSFRKKC(896) of HTNV, which locates on Gc, has been screened out from a set of 15-mer synthesized peptides covering the full-length of HTNV-GPs. It has been shown by the alanine-scanning technique that (885)C, (893)R, (894)K, (895)K and (896)C are the key amino acids of the binding sites of the GPs. The implications of identifying a novel B-cell epitope for hantavirus immunology and vaccinology are discussed.

PMID:
22933664
DOI:
10.1099/vir.0.045302-0
[Indexed for MEDLINE]

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