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PLoS One. 2011;6(8):e22365. doi: 10.1371/journal.pone.0022365. Epub 2011 Aug 4.

High-resolution description of antibody heavy-chain repertoires in humans.

Author information

1
Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts, United States of America.

Abstract

Antibodies' protective, pathological, and therapeutic properties result from their considerable diversity. This diversity is almost limitless in potential, but actual diversity is still poorly understood. Here we use deep sequencing to characterize the diversity of the heavy-chain CDR3 region, the most important contributor to antibody binding specificity, and the constituent V, D, and J segments that comprise it. We find that, during the stepwise D-J and then V-DJ recombination events, the choice of D and J segments exert some bias on each other; however, we find the choice of the V segment is essentially independent of both. V, D, and J segments are utilized with different frequencies, resulting in a highly skewed representation of VDJ combinations in the repertoire. Nevertheless, the pattern of segment usage was almost identical between two different individuals. The pattern of V, D, and J segment usage and recombination was insufficient to explain overlap that was observed between the two individuals' CDR3 repertoires. Finally, we find that while there are a near-infinite number of heavy-chain CDR3s in principle, there are about 3-9 million in the blood of an adult human being.

PMID:
21829618
PMCID:
PMC3150326
DOI:
10.1371/journal.pone.0022365
[Indexed for MEDLINE]
Free PMC Article

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