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J Pept Sci. 2011 Oct;17(10):708-14. doi: 10.1002/psc.1395. Epub 2011 Aug 1.

Conventional and microwave-assisted SPPS approach: a comparative synthesis of PTHrP(1-34)NH(2).

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Department of Chemistry Ugo Schiff, Laboratory of Peptide & Protein Chemistry & Biology, Via della Lastruccia 3/13, Polo Scientifico e Tecnologico, University of Florence, I-50019 Sesto Fiorentino, Italy.


Attracted by the possibility to optimize time and yield of the synthesis of difficult peptide sequences by MW irradiation, we compared Fmoc/tBu MW-assisted SPPS of 1-34 N-terminal fragment of parathyroid hormone-related peptide (PTHrP) with its conventional SPPS carried out at RT. MWs were applied in both coupling and deprotection steps of SPPS protocol. During the stepwise elongation of the resin-bound peptide, monitoring was conducted by performing MW-assisted mini-cleavages and analyzing them by UPLC-ESI-MS. Identification of some deletion sequences was helpful to recognize critical couplings and as such helped to guide the introduction of MW irradiations to these stages.

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