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BMC Microbiol. 2009 Mar 26;9:61. doi: 10.1186/1471-2180-9-61.

Staphylococcus aureus immunodominant surface antigen B is a cell-surface associated nucleic acid binding protein.

Author information

1
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298, USA. mackeynm@vcu.edu

Abstract

BACKGROUND:

Staphylococcus aureus immunodominant surface antigen B (IsaB) elicits an immune response during septicemia and is generally classified as a virulence factor, but its biological function remains completely undefined. In an attempt to identify staphylococcal RNA-binding proteins, we designed an RNA Affinity Chromatography assay and subsequently isolated IsaB.

RESULTS:

Western analysis indicated that IsaB was both secreted and cell-surface associated. Gel Shift analysis confirmed the RNA binding activity but revealed that IsaB bound to any nucleic acid without sequence specificity. IsaB exhibited the highest affinity for double-stranded DNA followed by single-stranded DNA and RNA. Because extracellular DNA has been shown to play a role in biofilm formation, we investigated the biofilm-forming capacity of an isogenic isaB deletion mutant but we found that IsaB did not contribute to biofilm formation under any conditions tested.

CONCLUSION:

IsaB is an extracellular nucleic acid binding protein, with little to no sequence specificity, but its role in virulence remains unclear.

PMID:
19323837
PMCID:
PMC2670837
DOI:
10.1186/1471-2180-9-61
[Indexed for MEDLINE]
Free PMC Article

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