Format

Send to

Choose Destination
J Virol. 2008 Sep;82(17):8942-6. doi: 10.1128/JVI.00676-08. Epub 2008 Jun 18.

Efficient multiplication of human metapneumovirus in Vero cells expressing the transmembrane serine protease TMPRSS2.

Author information

1
Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

Abstract

Human metapneumovirus (HMPV) is a major causative agent of severe bronchiolitis and pneumonia. Its fusion (F) protein must be cleaved by host proteases to cause membrane fusion, a critical step for virus infection. By generating Vero cells constitutively expressing the transmembrane serine protease TMPRSS2 and green fluorescent protein-expressing recombinant HMPV, we show that TMPRSS2, which is expressed in the human lung epithelium, cleaves the HMPV F protein efficiently and supports HMPV multiplication. The results indicate that TMPRSS2 is a possible candidate protease involved in the development of lower respiratory tract illness in HMPV-infected patients.

PMID:
18562527
PMCID:
PMC2519639
DOI:
10.1128/JVI.00676-08
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center