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Proc Natl Acad Sci U S A. 2007 Oct 23;104(43):17058-62. Epub 2007 Oct 11.

S-nitrosohemoglobin deficiency: a mechanism for loss of physiological activity in banked blood.

Author information

1
Department of Anesthesiology, Duke University Medical Center, Durham, NC 27710, USA.

Abstract

RBCs distribute oxygen to tissues, but, paradoxically, blood transfusion does not always improve oxygen delivery and is associated with ischemic events. We hypothesized that storage of blood would result in loss of NO bioactivity, impairing RBC vasodilation and thus compromising blood flow, and that repleting NO bioactivity would restore RBC function. We report that S-nitrosohemoglobin (SNO-Hb) concentrations declined rapidly after storage of fresh venous blood and that hypoxic vasodilation by banked RBCs correlated strongly with the amounts of SNO-Hb (r(2) = 0.90; P < 0.0005). Renitrosylation of banked blood during storage increased the SNO-Hb content and restored its vasodilatory activity. In addition, canine coronary blood flow was greater during infusion of renitrosylated RBCs than during infusion of S-nitrosothiol-depleted RBCs, and this difference in coronary flow was accentuated by hypoxemia (P < 0.001). Our findings indicate that NO bioactivity is depleted in banked blood, impairing the vasodilatory response to hypoxia, and they suggest that SNO-Hb repletion may improve transfusion efficacy.

PMID:
17940022
PMCID:
PMC2040473
DOI:
10.1073/pnas.0707958104
[Indexed for MEDLINE]
Free PMC Article

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