Format

Send to

Choose Destination
Neuron. 2007 Jul 5;55(1):25-36.

Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits.

Author information

1
Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA. kmicheva@stanford.edu

Erratum in

  • Neuron. 2007 Sep 6;55(5):824.

Abstract

Many biological functions depend critically upon fine details of tissue molecular architecture that have resisted exploration by existing imaging techniques. This is particularly true for nervous system tissues, where information processing function depends on intricate circuit and synaptic architectures. Here, we describe a new imaging method, called array tomography, which combines and extends superlative features of modern optical fluorescence and electron microscopy methods. Based on methods for constructing and repeatedly staining and imaging ordered arrays of ultrathin (50-200 nm), resin-embedded serial sections on glass microscope slides, array tomography allows for quantitative, high-resolution, large-field volumetric imaging of large numbers of antigens, fluorescent proteins, and ultrastructure in individual tissue specimens. Compared to confocal microscopy, array tomography offers the advantage of better spatial resolution, in particular along the z axis, as well as depth-independent immunofluorescent staining. The application of array tomography can reveal important but previously unseen features of brain molecular architecture.

PMID:
17610815
PMCID:
PMC2080672
DOI:
10.1016/j.neuron.2007.06.014
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center