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Appl Environ Microbiol. 2007 Sep;73(17):5653-6. Epub 2007 Jun 29.

Labeling of Bifidobacterium longum cells with 13C-substituted leucine for quantitative proteomic analyses.

Author information

1
Biomedical Proteomics Research Group, Department of Structural Biology and Bioinformatics, University of Geneva, 1 Rue Michel Servet, 1211 Geneva 14, Switzerland.

Abstract

Stable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [(13)C6]leucine. Using this strategy, proteins having variations of at least 50% in their expression rates can be quantified with great confidence.

PMID:
17601805
PMCID:
PMC2042066
DOI:
10.1128/AEM.00667-07
[Indexed for MEDLINE]
Free PMC Article

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