Format

Send to

Choose Destination
Apoptosis. 2007 Aug;12(8):1503-10.

Smac/DIABLO is required for effector caspase activation during apoptosis in human cells.

Author information

1
Department of Molecular Biology, Max Planck Institute for Infection Biology, Charit├ęplatz 1, 10117, Berlin, Germany.

Abstract

Mitochondria play a pivotal role during stress-induced apoptosis as several proapoptotic proteins are released to the cytosol to activate caspases. Smac/DIABLO is one of the proapoptotic proteins released from the mitochondria and has been shown to inactivate IAPs. However, gene knockout studies in mice revealed a redundant role for Smac during development and cell death. By applying RNA interference-mediated loss of function approach, we demonstrate that Smac/DIABLO is required for the activation of effector but not initiator caspases during stress and receptor-mediated cell death in HeLa cells. Cells with reduced Smac resist apoptosis and retained clonogenicity. Our results suggest an obligatory role for Smac/DIABLO in these tumor cells during several pathways of apoptosis induction.

PMID:
17440818
DOI:
10.1007/s10495-007-0067-7
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center