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Fertil Steril. 2007 Nov;88(5):1404-7. Epub 2007 Mar 26.

A comparison of blood spot vs. plasma analysis of gonadotropin and ovarian steroid hormone levels in reproductive-age women.

Author information

1
Department of Obstetrics and Gynecology, Oregon Health and Science University, Portland, Oregon 97239, USA. edelmana@ohsu.edu

Abstract

OBJECTIVE:

To compare LH, FSH, P, and E(2) levels obtained from blood spot vs. plasma (single-visit study) and to determine whether blood spots can document circulating hormone levels during ovulatory cycles (menstrual-cycle study).

DESIGN:

Cross-sectional study.

SETTING:

Academic center.

PATIENT(S):

Women 18-35 years of age with regular menstrual cycles and no recent use of hormonal contraception.

INTERVENTION(S):

Women contributed both a blood spot sample from a finger-stick and a plasma sample through venipuncture on a random day within their menstrual cycle (n = 100, single study visit). Five additional women were followed for an entire menstrual cycle with biweekly venipuncture and daily self-collected blood spot sampling. Samples were analyzed for FSH, LH, P, and E(2).

MAIN OUTCOME MEASURE(S):

Correlation between blood spot and plasma levels.

RESULT(S):

Significant positive correlations were found between the blood spot and plasma samples in the single-visit study (r(2): FSH, 0.91; LH, 0.93; P, 0.83; and E(2,) 0.70). Two of the 5 menstrual-cycle study women had ovulatory cycles based on P levels (>3 ng/mL) and an LH surge. Daily blood spot sampling was better able to document hormonal changes than biweekly venipuncture.

CONCLUSION(S):

Blood spot monitoring of FSH, LH, P, and, to a lesser extent, E(2), appears to be as valid as traditional plasma assays for clinical research and care.

PMID:
17368453
PMCID:
PMC2175208
DOI:
10.1016/j.fertnstert.2006.12.016
[Indexed for MEDLINE]
Free PMC Article

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