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J Clin Microbiol. 2007 Apr;45(4):1310-4. Epub 2007 Feb 7.

Characterization of class I Newcastle disease virus isolates from Hong Kong live bird markets and detection using real-time reverse transcription-PCR.

Author information

1
Southeast Poultry Research Laboratory, USDA-ARS, 934 College Station Rd., Athens, GA 30605, USA.

Abstract

Newcastle disease viruses isolated from Hong Kong live bird markets (LBMs) were not detected by a USDA-validated matrix gene real-time reverse transcription-PCR (RT-PCR) assay. Based upon phylogenetic analysis of the fusion gene, these viruses were related to lentogenic class I viruses found in U.S. LBMs and wild waterfowl. An alternative real-time RT-PCR assay which complements the matrix gene assay was developed to efficiently detect class I viruses.

PMID:
17287322
PMCID:
PMC1865838
DOI:
10.1128/JCM.02594-06
[Indexed for MEDLINE]
Free PMC Article

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