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J Proteome Res. 2006 Nov;5(11):2956-65.

Comparison of hydroxylated print additives on antibody microarray performance.

Author information

1
Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523-1872, USA.

Abstract

Various hydroxylated additives were added to antibody print buffers at different concentrations to stabilize printed antibodies during normal array spot desiccation on commercial polymer-coated microarray slides. Polyvinyl alcohol addition to print buffers produced the most regular spot morphologies, homogeneous intra-spot antibody distribution, uniform fluorescence intensity, and improved analyte capture activity, maintained up to 1 month at 4 degrees C for capturing model analytes, anti-human IL-1beta, IL-4, and TNFalpha, on these microarraying slides.

PMID:
17081047
PMCID:
PMC2528199
DOI:
10.1021/pr060217d
[Indexed for MEDLINE]
Free PMC Article

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