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Analyst. 2005 May;130(5):687-93. Epub 2005 Mar 22.

Construction of an electrochemical DNA chip for simultaneous genotyping of single nucleotide polymorphisms.

Author information

1
Toshiba Research & Development Center, 1, Komukai Toshiba-cho, Saiwai-ku, Kawasaki, Kanagawa Pref. 212-8582, Japan. masayoshi8.takahashi@toshiba.co.jp

Abstract

An electrochemical DNA chip was constructed for simultaneous genotyping of single nucleotide polymorphisms (SNPs) using genomic DNA extracted from blood samples. This chip consisted of electrodes located on a single piece of substrate and allele-specific oligonucleotide probes on the electrodes. As a first application, the 4 SNPs (MxA[-88], MxA[-123], MBL[X/Y], and MBL[A/B]), which have association with the efficacy of interferon therapy for HCV patient, were genotyped on the new DNA chip. Following hybridization of PCR products containing the 4 types of fragments, washing, bisbenzimide H33258 (Hoechst 33258) reaction and electrochemical analyses, 59 blood samples were genotyped by the chip method simultaneously. All procedures were completed within 2 h and the results were 100% concordant with those by the direct sequence method. The electrochemical DNA chip is expected to be a practical tool for SNPs genotyping.

PMID:
15852138
DOI:
10.1039/b414030n
[Indexed for MEDLINE]

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