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EMBO J. 2004 Dec 8;23(24):4868-75. Epub 2004 Nov 18.

The CDK regulates repair of double-strand breaks by homologous recombination during the cell cycle.

Author information

1
Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel.

Abstract

DNA double-strand breaks (DSBs) are dangerous lesions that can lead to genomic instability and cell death. Eukaryotic cells repair DSBs either by nonhomologous end-joining (NHEJ) or by homologous recombination. We investigated the ability of yeast cells (Saccharomyces cerevisiae) to repair a single, chromosomal DSB by recombination at different stages of the cell cycle. We show that cells arrested at the G1 phase of the cell cycle restrict homologous recombination, but are able to repair the DSB by NHEJ. Furthermore, we demonstrate that recombination ability does not require duplicated chromatids or passage through S phase, and is controlled at the resection step by Clb-CDK activity.

PMID:
15549137
PMCID:
PMC535085
DOI:
10.1038/sj.emboj.7600469
[Indexed for MEDLINE]
Free PMC Article

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