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J Chromatogr A. 2003 Sep 26;1013(1-2):157-71.

Capillary electrophoresis coupled to mass spectrometry to establish polypeptide patterns in dialysis fluids.

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Mosaiques Diagnostics and Therapeutics AG, Feodor-Lynen-Strasse 5, 30625 Hannover, Germany.


Combination of capillary electrophoresis with mass spectrometry (CE-MS) allows generation of polypeptide patterns of body fluids. In a single CE-MS (45 min) run more than 600 polypeptides were analyzed in hemodialysis fluids obtained with different membranes (high-flux/low-flux). Larger polypeptides (M(r) > 10 000) were almost exclusively present in high-flux dialysates only, while in low-flux dialysates additional small polypeptides were detected. Comparison to the normal urine pattern yielded a surprisingly low consensus: a number of polypeptides present in urine were missing. We established a fast and sensitive technique, easily applicable to the monitoring of different modalities of dialyzers.

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