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Biotechnology (N Y). 1991 Mar;9(3):273-8.

The functional expression of antibody Fv fragments in Escherichia coli: improved vectors and a generally applicable purification technique.

Author information

1
Genzentrum, Universität München, Max-Planck-Institut für Biochemie, Martinsried, FRG.

Abstract

We have previously demonstrated that the expression of fully functional Fv and Fab fragments in E. coli is possible by the simultaneous secretion of both chains to the periplasm. To increase production levels and facilitate engineering and random mutagenesis, we improved our previous vectors by introducing a resident repressor gene and a filamentous phage origin. We also developed a new purification strategy based on immobilized metal ion chromatography, with which a single-chain Fv fragment can be purified to homogeneity in a single step. We investigated the most efficient tail constructions and found that only a minimal structural change of three additional C-terminal amino acids is necessary. This modification has no deleterious effect on in vivo transport and folding or antigen affinity.

PMID:
1367302
[Indexed for MEDLINE]

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