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Can J Biochem. 1979 Mar;57(3):286-8.

A rapid method for the purification of antibody--enzyme conjugates.

Abstract

A method is described for a rapid and efficient separation of enzyme-labelled antibodies from the free enzyme following the coupling reaction. A single passage of the reaction mixture on a protein A--Sepharose CL-4B column gave a sharp separation of the free enzyme from the conjugate.

PMID:
108007
[Indexed for MEDLINE]

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