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Appl Environ Microbiol. 2012 Aug;78(16):5926-8. doi: 10.1128/AEM.00762-12. Epub 2012 Jun 1.

Exploiting a natural auxotrophy for genetic selection.

Author information

1
Department of Genome Sciences, University of Washington, Seattle, Washington, USA.

Abstract

We exploited the natural histidine auxotrophy of Francisella species to develop hisD (encodes histidinol dehydrogenase) as a positive selection marker. A shuttle plasmid (pBR103) carrying Escherichia coli hisD and designed for cloning of PCR fragments replicated in both attenuated and highly virulent Francisella strains. During this work, we formulated a simplified defined growth medium for Francisella novicida.

PMID:
22660718
PMCID:
PMC3406110
DOI:
10.1128/AEM.00762-12
[Indexed for MEDLINE]
Free PMC Article

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