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J Bacteriol. 2008 Jul;190(14):5137-41. doi: 10.1128/JB.00246-08. Epub 2008 May 16.

The Burkholderia mallei BmaR3-BmaI3 quorum-sensing system produces and responds to N-3-hydroxy-octanoyl homoserine lactone.

Author information

1
Department of Microbiology, University of Washington School of Medicine, Seattle, Washington 98195-7242, USA.

Abstract

Burkholderia mallei has two acyl-homoserine lactone (acyl-HSL) signal generator-receptor pairs and two additional signal receptors, all of which contribute to virulence. We show that B. mallei produces N-3-hydroxy-octanoyl HSL (3OHC8-HSL) but a bmaI3 mutant does not. Recombinant Escherichia coli expressing BmaI3 produces hydroxylated acyl-HSLs, with 3OHC8-HSL being the most abundant compound. In recombinant E. coli, BmaR3 responds to 3OHC8-HSL but not to other acyl-HSLs. These data indicate that the signal for BmaR3-BmaI3 quorum sensing is 3OHC8-HSL.

PMID:
18487338
PMCID:
PMC2447019
DOI:
10.1128/JB.00246-08
[Indexed for MEDLINE]
Free PMC Article

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