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Items: 1 to 20 of 166

1.

Integrated Extreme Real-Time PCR and High-Speed Melting Analysis in 52 to 87 Seconds.

Myrick JT, Pryor RJ, Palais RA, Ison SJ, Sanford L, Dwight ZL, Huuskonen JJ, Sundberg SO, Wittwer CT.

Clin Chem. 2018 Nov 20. pii: clinchem.2018.296608. doi: 10.1373/clinchem.2018.296608. [Epub ahead of print]

PMID:
30459167
2.

A "Culture" Shift: Broad Bacterial Detection, Identification, and Antimicrobial Susceptibility Testing Directly from Whole Blood.

Andini N, Hu A, Zhou L, Cogill S, Wang TH, Wittwer CT, Yang S.

Clin Chem. 2018 Oct;64(10):1453-1462. doi: 10.1373/clinchem.2018.290189. Epub 2018 Aug 7.

3.

The Effect of Single Mismatches on Primer Extension.

Rejali NA, Moric E, Wittwer CT.

Clin Chem. 2018 May;64(5):801-809. doi: 10.1373/clinchem.2017.282285. Epub 2018 Feb 14.

PMID:
29444902
4.

Quantifying variant differences in DNA melting curves: Effects of length, melting rate, and curve overlay.

Li M, Palais RA, Zhou L, Wittwer CT.

Anal Biochem. 2017 Dec 15;539:90-95. doi: 10.1016/j.ab.2017.10.015. Epub 2017 Oct 21.

PMID:
29061329
5.

High-Speed Melting Analysis: The Effect of Melting Rate on Small Amplicon Microfluidic Genotyping.

Pryor RJ, Myrick JT, Palais RA, Sundberg SO, Paek JY, Wittwer CT, Knight IT.

Clin Chem. 2017 Oct;63(10):1624-1632. doi: 10.1373/clinchem.2017.276147. Epub 2017 Aug 17.

6.

MIQE: A Step Toward More Robust and Reproducible Quantitative PCR.

Bustin SA, Wittwer CT.

Clin Chem. 2017 Sep;63(9):1537-1538. doi: 10.1373/clinchem.2016.268953. Epub 2017 Jun 12. Review. No abstract available.

7.

Gain-of-function EGLN1 prolyl hydroxylase (PHD2 D4E:C127S) in combination with EPAS1 (HIF-2α) polymorphism lowers hemoglobin concentration in Tibetan highlanders.

Tashi T, Scott Reading N, Wuren T, Zhang X, Moore LG, Hu H, Tang F, Shestakova A, Lorenzo F, Burjanivova T, Koul P, Guchhait P, Wittwer CT, Julian CG, Shah B, Huff CD, Gordeuk VR, Prchal JT, Ge R.

J Mol Med (Berl). 2017 Jun;95(6):665-670. doi: 10.1007/s00109-017-1519-3. Epub 2017 Feb 23.

PMID:
28233034
8.

Democratizing the Real-Time PCR.

Wittwer CT.

Clin Chem. 2017 Apr;63(4):924-925. doi: 10.1373/clinchem.2016.263269. Epub 2017 Feb 3. No abstract available.

9.

Portable Nanopore Sequencing for Viral Surveillance.

Wittwer CT.

Clin Chem. 2016 Nov;62(11):1427-1429. Epub 2016 Sep 16. No abstract available.

10.

Genomic Copy Number Analysis of HER2-Equivocal Breast Cancers.

Geiersbach KB, Willmore-Payne C, Pasi AV, Paxton CN, Werner TL, Xu X, Wittwer CT, Gulbahce HE, Downs-Kelly E.

Am J Clin Pathol. 2016 Oct;146(4):439-47. doi: 10.1093/ajcp/aqw130. Epub 2016 Sep 10.

PMID:
27614666
11.

Disruptive Innovation in Laboratory Medicine.

Rifai N, Topol E, Chan E, Lo YM, Wittwer CT.

Clin Chem. 2015 Sep;61(9):1129-32. doi: 10.1373/clinchem.2015.243667. Epub 2015 Jul 7. No abstract available.

12.

Detection of chromosomal translocations in formalin-fixed paraffin-embedded (FFPE) leukemic specimens by digital expression profiling.

Liew M, Mao R, Wittwer CT, Salama ME.

Int J Lab Hematol. 2015 Oct;37(5):690-8. doi: 10.1111/ijlh.12388. Epub 2015 Jun 3.

PMID:
26037935
13.

Copy number assessment by competitive PCR with limiting deoxynucleotide triphosphates and high-resolution melting.

Zhou L, Palais RA, Paxton CN, Geiersbach KB, Wittwer CT.

Clin Chem. 2015 May;61(5):724-33. doi: 10.1373/clinchem.2014.236208. Epub 2015 Mar 10.

14.

Rare event counting of CD59- red cells in human blood: A 47-month experience using PNH consensus guidelines for WBC and RBC testing in a reference lab.

Liew M, Farley M, Andreasen J, Parker CJ, Wittwer CT.

Cytometry B Clin Cytom. 2015 Jul-Aug;88(4):261-9. doi: 10.1002/cyto.b.21225. Epub 2015 Feb 3.

15.

Molecular diagnostics: a revolution in progress.

Chiu RW, Lo YM, Wittwer CT.

Clin Chem. 2015 Jan;61(1):1-3. doi: 10.1373/clinchem.2014.233361. No abstract available.

16.

Extreme PCR: efficient and specific DNA amplification in 15-60 seconds.

Farrar JS, Wittwer CT.

Clin Chem. 2015 Jan;61(1):145-53. doi: 10.1373/clinchem.2014.228304. Epub 2014 Oct 15.

17.

Microfluidic genotyping by rapid serial PCR and high-speed melting analysis.

Sundberg SO, Wittwer CT, Howell RM, Huuskonen J, Pryor RJ, Farrar JS, Stiles HM, Palais RA, Knight IT.

Clin Chem. 2014 Oct;60(10):1306-13. doi: 10.1373/clinchem.2014.223768. Epub 2014 Aug 12.

18.

Quasi-digital PCR: Enrichment and quantification of rare DNA variants.

Sundberg SO, Wittwer CT, Zhou L, Palais R, Dwight Z, Gale BK.

Biomed Microdevices. 2014 Aug;16(4):639-44. doi: 10.1007/s10544-014-9866-0.

PMID:
24781884
19.

Genotyping accuracy of high-resolution DNA melting instruments.

Li M, Zhou L, Palais RA, Wittwer CT.

Clin Chem. 2014 Jun;60(6):864-72. doi: 10.1373/clinchem.2013.220160. Epub 2014 Apr 9.

20.

The influence of nucleotide sequence and temperature on the activity of thermostable DNA polymerases.

Montgomery JL, Rejali N, Wittwer CT.

J Mol Diagn. 2014 May;16(3):305-13. doi: 10.1016/j.jmoldx.2014.01.006. Epub 2014 Mar 6.

PMID:
24607271

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