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Items: 7

1.

Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies.

Aceti DJ, Bingman CA, Wrobel RL, Frederick RO, Makino S, Nichols KW, Sahu SC, Bergeman LF, Blommel PG, Cornilescu CC, Gromek KA, Seder KD, Hwang S, Primm JG, Sabat G, Vojtik FC, Volkman BF, Zolnai Z, Phillips GN Jr, Markley JL, Fox BG.

J Struct Funct Genomics. 2015 Jun;16(2):67-80. doi: 10.1007/s10969-015-9198-1. Epub 2015 Apr 9.

2.

The oligomeric states of the purified sigma-1 receptor are stabilized by ligands.

Gromek KA, Suchy FP, Meddaugh HR, Wrobel RL, LaPointe LM, Chu UB, Primm JG, Ruoho AE, Senes A, Fox BG.

J Biol Chem. 2014 Jul 18;289(29):20333-44. doi: 10.1074/jbc.M113.537993. Epub 2014 May 20.

3.

Improved expression and purification of sigma 1 receptor fused to maltose binding protein by alteration of linker sequence.

Gromek KA, Meddaugh HR, Wrobel RL, Suchy FP, Bingman CA, Primm JG, Fox BG.

Protein Expr Purif. 2013 Jun;89(2):203-9. doi: 10.1016/j.pep.2013.03.013. Epub 2013 Apr 3.

4.

Robotic large-scale application of wheat cell-free translation to structural studies including membrane proteins.

Beebe ET, Makino S, Nozawa A, Matsubara Y, Frederick RO, Primm JG, Goren MA, Fox BG.

N Biotechnol. 2011 Apr 30;28(3):239-49. doi: 10.1016/j.nbt.2010.07.003. Epub 2010 Jul 15.

5.

The Center for Eukaryotic Structural Genomics.

Markley JL, Aceti DJ, Bingman CA, Fox BG, Frederick RO, Makino S, Nichols KW, Phillips GN Jr, Primm JG, Sahu SC, Vojtik FC, Volkman BF, Wrobel RL, Zolnai Z.

J Struct Funct Genomics. 2009 Apr;10(2):165-79. doi: 10.1007/s10969-008-9057-4. Epub 2009 Jan 8.

6.

Small-scale, semi-automated purification of eukaryotic proteins for structure determination.

Frederick RO, Bergeman L, Blommel PG, Bailey LJ, McCoy JG, Song J, Meske L, Bingman CA, Riters M, Dillon NA, Kunert J, Yoon JW, Lim A, Cassidy M, Bunge J, Aceti DJ, Primm JG, Markley JL, Phillips GN Jr, Fox BG.

J Struct Funct Genomics. 2007 Dec;8(4):153-66. Epub 2007 Nov 6.

7.

Inexpensive manual synthesis of antithrombotic peptides.

Primm JG, Kunicki TJ.

Nouv Rev Fr Hematol. 1992;34(1):141-5.

PMID:
1523095

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