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Year Number of Results
1963 2
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1965 1
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1970 12
1971 10
1972 34
1973 60
1974 96
1975 193
1976 222
1977 359
1978 477
1979 695
1980 901
1981 1133
1982 1505
1983 1862
1984 2206
1985 2306
1986 2343
1987 2570
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1989 3406
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1991 3704
1992 3553
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1998 2785
1999 2924
2000 3328
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2002 3412
2003 3753
2004 3935
2005 3787
2006 3301
2007 3133
2008 3060
2009 2841
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2011 3043
2012 3166
2013 2833
2014 2605
2015 2449
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2017 2105
2018 1986
2019 1921
2020 1840
2021 1673
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2024 413

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114,667 results

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Isolation of plasmid DNA from bacteria.
Andreou LV. Andreou LV. Methods Enzymol. 2013;529:135-42. doi: 10.1016/B978-0-12-418687-3.00010-0. Methods Enzymol. 2013. PMID: 24011041
The purpose of this protocol is the isolation of plasmid DNA from bacteria. The boiling method for isolating plasmids by Holmes and Quigley (1981) is presented here. This method is rapid and simple and it allows for a large number of samples to be processed s …
The purpose of this protocol is the isolation of plasmid DNA from bacteria. The boiling method for isolating plasmids b …
A scalable, extrusion-free method for efficient liposomal encapsulation of plasmid DNA.
Jeffs LB, Palmer LR, Ambegia EG, Giesbrecht C, Ewanick S, MacLachlan I. Jeffs LB, et al. Pharm Res. 2005 Mar;22(3):362-72. doi: 10.1007/s11095-004-1873-z. Pharm Res. 2005. PMID: 15835741
METHODS: Liposomes encapsulating plasmid DNA were formed instantaneously by mixing lipids dissolved in ethanol with an aqueous solution of DNA in a controlled, stepwise manner. ...The resulting DNA-containing liposomes were further stabilized by a seco …
METHODS: Liposomes encapsulating plasmid DNA were formed instantaneously by mixing lipids dissolved in ethanol with an aqueous …
Plasmid DNA manufacturing technology.
Carnes AE, Williams JA. Carnes AE, et al. Recent Pat Biotechnol. 2007;1(2):151-66. doi: 10.2174/187220807780809436. Recent Pat Biotechnol. 2007. PMID: 19075838 Review.
Today, plasmid DNA is becoming increasingly important as the next generation of biotechnology products (gene medicines and DNA vaccines) make their way into clinical trials, and eventually into the pharmaceutical marketplace. ...Strategies for plasmid …
Today, plasmid DNA is becoming increasingly important as the next generation of biotechnology products (gene medicines and …
Production of Plasmid DNA as Pharmaceutical.
Schmeer M, Schleef M. Schmeer M, et al. Methods Mol Biol. 2015;1317:315-26. doi: 10.1007/978-1-4939-2727-2_17. Methods Mol Biol. 2015. PMID: 26072414 Review.
Pharmaceutical applications of plasmid DNA require certain quality standards, depending on the intended use of the plasmids. That is, for direct gene transfer into human, GMP Grade is mandatory, however, for GMP production of for example viral vectors (AAV or …
Pharmaceutical applications of plasmid DNA require certain quality standards, depending on the intended use of the plasmids …
Topological Behavior of Plasmid DNA.
Higgins NP, Vologodskii AV. Higgins NP, et al. Microbiol Spectr. 2015 Apr;3(2):10.1128/microbiolspec.PLAS-0036-2014. doi: 10.1128/microbiolspec.PLAS-0036-2014. Microbiol Spectr. 2015. PMID: 26104708 Free PMC article. Review.
The emphasis is on the power of plasmids for studying DNA structure and function. The conditions that trigger the formation of alternative DNA structures such as left-handed Z-DNA, inter- and intra-molecular triplexes, triple-stranded DNA, and l …
The emphasis is on the power of plasmids for studying DNA structure and function. The conditions that trigger the formation of …
[Plasmid DNA extraction by double magnetic beads method and its application].
Yu C, Song H, Cao W, Xiao X, DU J. Yu C, et al. Sheng Wu Gong Cheng Xue Bao. 2023 Nov 25;39(11):4708-4717. doi: 10.13345/j.cjb.230277. Sheng Wu Gong Cheng Xue Bao. 2023. PMID: 38013194 Free article. Chinese.
The effects of the input of magnetic beads, the size of plasmid DNA fragments, and the volume of bacterial on plasmid DNA extraction were explored. In addition, the quality, throughput, and cost of plasmid DNA extraction were also compare …
The effects of the input of magnetic beads, the size of plasmid DNA fragments, and the volume of bacterial on plasmid …
Plasmid segregation: how to survive as an extra piece of DNA.
Salje J. Salje J. Crit Rev Biochem Mol Biol. 2010 Aug;45(4):296-317. doi: 10.3109/10409238.2010.494657. Crit Rev Biochem Mol Biol. 2010. PMID: 20586577 Review.
A comparison of different plasmid segregation systems reveals (i) how unrelated filament-forming and DNA-binding proteins have been adopted and modified to create a range of simple DNA segregating complexes and (ii) how subtle changes in the few components of …
A comparison of different plasmid segregation systems reveals (i) how unrelated filament-forming and DNA-binding proteins have …
A method for plasmid purification directly from yeast.
Singh MV, Weil PA. Singh MV, et al. Anal Biochem. 2002 Aug 1;307(1):13-7. doi: 10.1016/s0003-2697(02)00018-0. Anal Biochem. 2002. PMID: 12137773
A rapid technique for purifying plasmids from yeast Saccharomyces cerevisiae is described that yields high-quality DNA suitable for bacterial transformation, yeast transformation, and direct DNA sequencing. ...Both yeast single-copy CEN/ARS and high-copy 2mic …
A rapid technique for purifying plasmids from yeast Saccharomyces cerevisiae is described that yields high-quality DNA suitabl …
Preparation of bacterial plasmid DNA.
Engebrecht J, Heilig JS, Brent R. Engebrecht J, et al. Curr Protoc Immunol. 2001 May;Chapter 10:Unit 10.3. doi: 10.1002/0471142735.im1003s27. Curr Protoc Immunol. 2001. PMID: 18432694 Review.
The protocols in this unit describe methods for preparing bacterial plasmid DNA free from chromosomal DNA. The first is an alkaline lysis miniprep suitable for screening a moderate number of bacterial colonies by restriction endonuclease cleavage and agarose …
The protocols in this unit describe methods for preparing bacterial plasmid DNA free from chromosomal DNA. The first is …
Effects of polyamines on the ultrafiltration of plasmid DNA.
Li Y, Manzano I, Zydney AL. Li Y, et al. Biotechnol Prog. 2019 Mar;35(2):e2765. doi: 10.1002/btpr.2765. Epub 2018 Dec 24. Biotechnol Prog. 2019. PMID: 30537363
It is well established that the structure of plasmid DNA is a strong function of solution ionic conditions due to changes in intramolecular electrostatic interactions between the charged phosphate groups along the DNA backbone. Multivalent cations like spermi …
It is well established that the structure of plasmid DNA is a strong function of solution ionic conditions due to changes in i …
114,667 results
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