Format

Send to

Choose Destination

See 1 citation found by title matching your search:

See comment in PubMed Commons below
Mol Neurobiol. 2016 Sep;53(7):4701-12. doi: 10.1007/s12035-015-9383-z. Epub 2015 Aug 29.

miR-132 Regulates Dendritic Spine Structure by Direct Targeting of Matrix Metalloproteinase 9 mRNA.

Author information

  • 1Laboratory of Neurobiology, The Nencki Institute, Pasteura 3, 02-093, Warsaw, Poland.
  • 2Laboratory of Molecular Basis of Synaptic Plasticity, Center of New Technologies, University of Warsaw, Warsaw, Poland.
  • 3School of Molecular Medicine, Żwirki i Wigury 61, 02-091, Warsaw, Poland.
  • 4Institute of Biochemistry and Biophysics, 02-106, Warsaw, Poland.
  • 5Laboratory of Molecular and Cellular Neurobiology, The International Institute of Molecular and Cell Biology, Warsaw, Poland.
  • 6Laboratory of Neuroinformatics, The Nencki Institute, Pasteura 3, 02-093, Warsaw, Poland.
  • 7Laboratory of Neurobiology, The Nencki Institute, Pasteura 3, 02-093, Warsaw, Poland. l.kaczmarek@nencki.gov.pl.
  • 8Laboratory of Neurobiology, The Nencki Institute, Pasteura 3, 02-093, Warsaw, Poland. m.dziembowska@nencki.gov.pl.
  • 9Laboratory of Molecular Basis of Synaptic Plasticity, Center of New Technologies, University of Warsaw, Warsaw, Poland. m.dziembowska@nencki.gov.pl.

Abstract

Mir-132 is a neuronal activity-regulated microRNA that controls the morphology of dendritic spines and neuronal transmission. Similar activities have recently been attributed to matrix metalloproteinase-9 (MMP-9), an extrasynaptic protease. In the present study, we provide evidence that miR-132 directly regulates MMP-9 mRNA in neurons to modulate synaptic plasticity. With the use of luciferase reporter system, we show that miR-132 binds to the 3'UTR of MMP-9 mRNA to regulate its expression in neurons. The overexpression of miR-132 in neurons reduces the level of endogenous MMP-9 protein secretion. In synaptoneurosomes, metabotropic glutamate receptor (mGluR)-induced signaling stimulates the dissociation of miR-132 from polyribosomal fractions and shifts it towards the messenger ribonucleoprotein (mRNP)-containing fraction. Furthermore, we demonstrate that the overexpression of miR-132 in the cultured hippocampal neurons from Fmr1 KO mice that have increased synaptic MMP-9 level provokes enlargement of the dendritic spine heads, a process previously implicated in enhanced synaptic plasticity. We propose that activity-dependent miR-132 regulates structural plasticity of dendritic spines through matrix metalloproteinase 9.

KEYWORDS:

Matrix metalloproteinase 9 (MMP-9); Structural plasticity of dendritic spines; miR-132

PMID:
26319558
PMCID:
PMC4965491
DOI:
10.1007/s12035-015-9383-z
[PubMed - in process]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer Icon for PubMed Central
    Loading ...
    Support Center