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See 1 citation in Nature Methods 2015 by Lacava:

Nat Methods. 2015 Jun;12(6):553-60. doi: 10.1038/nmeth.3395. Epub 2015 May 4.

Rapid, optimized interactomic screening.

Author information

1
Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, New York, USA.
2
1] Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, New York, USA. [2] Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
3
Orochem Technologies Inc., Naperville, Illinois, USA.
4
1] Center for Health Informatics and Bioinformatics, New York University School of Medicine, New York, New York, USA. [2] Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, New York, USA.
5
1] Institute for Systems Biology, Seattle, Washington, USA. [2] Seattle Biomedical Research Institute, Seattle, Washington, USA.
6
Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York, USA.
7
High Energy Physics Instrument Shop, The Rockefeller University, New York, New York, USA.
8
Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.

Abstract

We must reliably map the interactomes of cellular macromolecular complexes in order to fully explore and understand biological systems. However, there are no methods to accurately predict how to capture a given macromolecular complex with its physiological binding partners. Here, we present a screening method that comprehensively explores the parameters affecting the stability of interactions in affinity-captured complexes, enabling the discovery of physiological binding partners in unparalleled detail. We have implemented this screen on several macromolecular complexes from a variety of organisms, revealing novel profiles for even well-studied proteins. Our approach is robust, economical and automatable, providing inroads to the rigorous, systematic dissection of cellular interactomes.

PMID:
25938370
PMCID:
PMC4449307
DOI:
10.1038/nmeth.3395
[Indexed for MEDLINE]
Free PMC Article

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