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Biochem Biophys Res Commun. 2009 Oct 9;388(1):117-21. doi: 10.1016/j.bbrc.2009.07.130. Epub 2009 Jul 30.

A role for AMPK in the inhibition of glucose-6-phosphate dehydrogenase by polyunsaturated fatty acids.

Author information

1
Department of Biochemistry, West Virginia University, Morgantown, 26506, USA.

Abstract

Both polyunsaturated fatty acids and AMPK promote energy partitioning away from energy consuming processes, such as fatty acid synthesis, towards energy generating processes, such as beta-oxidation. In this report, we demonstrate that arachidonic acid activates AMPK in primary rat hepatocytes, and that this effect is p38 MAPK-dependent. Activation of AMPK mimics the inhibition by arachidonic acid of the insulin-mediated induction of G6PD. Similar to intracellular signaling by arachidonic acid, AMPK decreases insulin signal transduction, increasing Ser(307) phosphorylation of IRS-1 and a subsequent decrease in AKT phosphorylation. Overexpression of dominant-negative AMPK abolishes the effect of arachidonic acid on G6PD expression. These data suggest a role for AMPK in the inhibition of G6PD by polyunsaturated fatty acids.

PMID:
19646964
PMCID:
PMC2756033
DOI:
10.1016/j.bbrc.2009.07.130
[Indexed for MEDLINE]
Free PMC Article

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