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Dent Mater. 2017 Jun;33(6):743-751. doi: 10.1016/j.dental.2017.04.007. Epub 2017 Apr 29.

Unbound monomers do diffuse through the dentin barrier.

Author information

1
Unité de Recherche en Biomatériaux Innovants et Interfaces (URB2I)-EA4462, Faculté de Chirurgie Dentaire, Université Paris Descartes, Sorbonne Paris Cité, 1 rue Maurice Arnoux, 92120 Montrouge, France.
2
Laboratoire de Physiopathologie Orale Moléculaire INSERM UMR S1138, Centre de Recherche des Cordeliers, Universités Paris-Diderot et Paris-Descartes, 15 rue de l'Ecole de Médecine, 75006 Paris, France.
3
Unité de Recherche en Biomatériaux Innovants et Interfaces (URB2I)-EA4462, Faculté de Chirurgie Dentaire, Université Paris Descartes, Sorbonne Paris Cité, 1 rue Maurice Arnoux, 92120 Montrouge, France. Electronic address: mathieu_derbanne@hotmail.fr.

Abstract

OBJECTIVES:

Assessing the role of dentinal fluid proteins in trans-dentinal diffusion of free monomers in vitro.

METHODS:

An artificial pulp chamber (APC) topped human dentin disks was used. A simplified two-step etch-and-rinse adhesive was formulated with 2-hydroethyl-methacrylate (HEMA), Bisphenol-A-diglycidyl-methacrylate (BisGMA), using Camphorquinone/tertiary amine as initiators. Two extraction media were used: buffered saline (Control), buffered saline with 1% bovine serum albumin (BSA). Samples were acid-etched, rinsed, air dried. Simplified primer was used, adhesive applied then light cured with a LED curing. Monomer diffusion was assessed by reverse phase HPLC.

RESULTS:

Quantifiable amounts of HEMA were detected in both extraction media while BisGMA was present in quantifiable amounts in BSA medium only. Diffused monomers concentrations were significantly higher for both monomers in BSA extraction medium.

SIGNIFICANCE:

Albumin is sometimes referred to as taxi protein for its ability to bind and transport hydrophobic ligands. From our results, we hypothesized that albumin can also transport unbound monomers released from dental adhesive through the dentin barrier. However, dentinal fluid proteins like albumin could have significant effect on monomer diffusion through dentin to the dental pulp transporting highly hydrophobic molecules like BisGMA and enhancing diffusion of more hydrophilic ones like HEMA. These results demonstrate a new possible mechanism for cytotoxicity of resin monomers.

KEYWORDS:

Albumin; Biocompatibility; Cytotoxicity; Dental adhesives; Dentin; Monomers; Pulp

PMID:
28465067
DOI:
10.1016/j.dental.2017.04.007
[Indexed for MEDLINE]

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