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Clin Chim Acta. 2005 Aug;358(1-2):139-45.

Effect of buformin and metformin on formation of advanced glycation end products by methylglyoxal.

Author information

1
Gifu Prefectural Institute of Health and Environmental Sciences, 1-1 Naka-fudogaoka, Kakamigahara 504-0838, Japan. kiho-tadashi@pref.gifu.lg.jp

Abstract

BACKGROUND:

The formation and accumulation of advanced glycation end products (AGE) in various tissues are known to be involved in the aging process and complications of long-term diabetes. Aminoguanidine as AGE inhibitors was first studied, and metformin as biguanide compounds have been reported to react with reactive dicarbonyl precursors such as methylglyoxal.

METHODS:

We studied the effects of the biguanides of buformin and metformin on AGE formation by the methods of specific fluorescence, and enzyme-linked immunosorbent assay and a Western blot analysis using the anti-AGE antibody after incubating BSA or RNase with methylglyoxal.

RESULTS:

Buformin is a more potent inhibitor of AGE formation than metformin, and suggests that the amino group of buformin trap the carbonyl group of methylglyoxal to suppress formation of AGE.

CONCLUSION:

In addition to that of metformin, buformin may be clinically useful to prevent diabetic complications.

PMID:
15946656
DOI:
10.1016/j.cccn.2005.02.012
[Indexed for MEDLINE]

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