Send to

Choose Destination

[Screening and identification of differential expression genes related to stress fracture by cDNA microarray assay].

[Article in Chinese]

Author information

Key Laboratory of Biomarkers for Occupational and Environmental Hazard, Medical College of Chinese People's Armed Police Forces, Tianjin 300162, China.



To evaluate the differentially expressed genes between the Stress fracture (SF) cases and controls.


Total RNA was extracted and purified from peripheral blood sample of 3 SF cases and 3 controls who conducted a 1:1 matched case-control study, then used for Human Genome Array analysis. The hybridization data were analyzed using SAM software. Parts of these genes were analyzed and identified by real-time PCR.


Upregulated and downregulated genes were 22 and 1, respectively. Thus the highest ratio and most significant cytokine was tumor necrosis factor receptor superfamily, member 10c (TNFRSF10C). The result of real-time PCR shows that TNFRSF10C was over-expressed in 3 cases and low-expressed in 1 case.


Obvious difference exists in gene expression between SF cases and controls, showing there may be a lot of genes involving in the occurrence and development of SF. Meanwhile, the identification of the specific genes is helpful for biomechanics study, early diagnosis and screening of SF.

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center