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Biotechnol Bioeng. 1993 May;41(11):1112-20.

Mathematical model for the luminol chemiluminescence reaction catalyzed by peroxidase.

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1
Department of Chemistry, University of lowa City, lowa 52242, USA.

Abstract

A kinetic model that accurately describes intensity vs. time reaction profiles for the chemiluminescence reaction between luminol and hydrogen peroxide, as catalyzed by horseradish peroxidase, is derived and evaluated. A set of three differential equations is derived and solved to provide intensity time information for the first 200 seconds of the reaction. The model accurately predicts intensity-time profiles when literature values are used for all but one of the reaction rate constants. Furthermore, the model predicts a nonlinear curve for plots of light intensity versus the initial hydrogen peroxide concentration. Experimental data confirm that such plots are nonlinear. Finally, a linear double-reciprocal plot is predicted by the model and the experimental data verify this relationship.

PMID:
18601298
DOI:
10.1002/bit.260411115

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