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Metagenomics of extreme environments.
Cowan DA, Ramond JB, Makhalanyane TP, De Maayer P. Cowan DA, et al. Curr Opin Microbiol. 2015 Jun;25:97-102. doi: 10.1016/j.mib.2015.05.005. Epub 2015 Jun 2. Curr Opin Microbiol. 2015. PMID: 26048196 Review.
This ability to survive has fascinated scientists for nearly a century, but the recent development of metagenomics and 'omics' tools has allowed us to make huge leaps in understanding the remarkable complexity and versatility of extremophile communities. Here, in the conte …
This ability to survive has fascinated scientists for nearly a century, but the recent development of metagenomics and 'omics' tools …
Metagenomic analysis provides insights into functional capacity in a hyperarid desert soil niche community.
Vikram S, Guerrero LD, Makhalanyane TP, Le PT, Seely M, Cowan DA. Vikram S, et al. Environ Microbiol. 2016 Jun;18(6):1875-88. doi: 10.1111/1462-2920.13088. Epub 2015 Dec 10. Environ Microbiol. 2016. PMID: 26470632
While hypolithic communities are considered to play a major role in productivity, the functional guilds implicated in these processes remain unclear. Here, we describe the metagenomic sequencing, assembly and analysis of hypolithic microbial communities from the Namib Dese …
While hypolithic communities are considered to play a major role in productivity, the functional guilds implicated in these processes remain …
A novel bacterial Water Hypersensitivity-like protein shows in vivo protection against cold and freeze damage.
Anderson D, Ferreras E, Trindade M, Cowan D. Anderson D, et al. FEMS Microbiol Lett. 2015 Aug;362(15):fnv110. doi: 10.1093/femsle/fnv110. Epub 2015 Jul 17. FEMS Microbiol Lett. 2015. PMID: 26187747
Metagenomic library screening, by functional or sequence analysis, has become an established method for the identification of novel genes and gene products, including genetic elements implicated in microbial stress response and adaptation. We have identified, using a seque
Metagenomic library screening, by functional or sequence analysis, has become an established method for the identification of novel g
Normalization of environmental metagenomic DNA enhances the discovery of under-represented microbial community members.
Ramond JB, Makhalanyane TP, Tuffin MI, Cowan DA. Ramond JB, et al. Lett Appl Microbiol. 2015 Apr;60(4):359-66. doi: 10.1111/lam.12380. Epub 2015 Jan 15. Lett Appl Microbiol. 2015. PMID: 25495929
Here, we present a normalization protocol involving the direct treatment of extracted environmental metagenomic DNA with S1 nuclease, referred to as normalization of metagenomic DNA: NmDNA. ...SIGNIFICANCE AND IMPACT OF THE STUDY: This study is the first testing 'no …
Here, we present a normalization protocol involving the direct treatment of extracted environmental metagenomic DNA with S1 nuclease, …