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Items: 1 to 20 of 21

1.

The cAMP-induced G protein subunits dissociation monitored in live Dictyostelium cells by BRET reveals two activation rates, a positive effect of caffeine and potential role of microtubules.

Tariqul Islam AFM, Yue H, Scavello M, Haldeman P, Rappel WJ, Charest PG.

Cell Signal. 2018 Aug;48:25-37. doi: 10.1016/j.cellsig.2018.04.005. Epub 2018 Apr 24.

PMID:
29698704
2.

Protein kinase A regulates the Ras, Rap1 and TORC2 pathways in response to the chemoattractant cAMP in Dictyostelium.

Scavello M, Petlick AR, Ramesh R, Thompson VF, Lotfi P, Charest PG.

J Cell Sci. 2017 May 1;130(9):1545-1558. doi: 10.1242/jcs.177170. Epub 2017 Mar 16.

3.

Studying Chemoattractant Signal Transduction Dynamics in Dictyostelium by BRET.

Islam AF, Stepanski BM, Charest PG.

Methods Mol Biol. 2016;1407:63-77. doi: 10.1007/978-1-4939-3480-5_5.

PMID:
27271894
4.

The small GTPases Ras and Rap1 bind to and control TORC2 activity.

Khanna A, Lotfi P, Chavan AJ, Montaño NM, Bolourani P, Weeks G, Shen Z, Briggs SP, Pots H, Van Haastert PJ, Kortholt A, Charest PG.

Sci Rep. 2016 May 13;6:25823. doi: 10.1038/srep25823.

5.

Degradation of activated K-Ras orthologue via K-Ras-specific lysine residues is required for cytokinesis.

Sumita K, Yoshino H, Sasaki M, Majd N, Kahoud ER, Takahashi H, Takeuchi K, Kuroda T, Lee S, Charest PG, Takeda K, Asara JM, Firtel RA, Anastasiou D, Sasaki AT.

J Biol Chem. 2014 Feb 14;289(7):3950-9. doi: 10.1074/jbc.M113.531178. Epub 2013 Dec 13.

6.

Daydreamer, a Ras effector and GSK-3 substrate, is important for directional sensing and cell motility.

Kölsch V, Shen Z, Lee S, Plak K, Lotfi P, Chang J, Charest PG, Romero JL, Jeon TJ, Kortholt A, Briggs SP, Firtel RA.

Mol Biol Cell. 2013 Jan;24(2):100-14. doi: 10.1091/mbc.E12-04-0271. Epub 2012 Nov 7.

7.

Incoherent feedforward control governs adaptation of activated ras in a eukaryotic chemotaxis pathway.

Takeda K, Shao D, Adler M, Charest PG, Loomis WF, Levine H, Groisman A, Rappel WJ, Firtel RA.

Sci Signal. 2012 Jan 3;5(205):ra2. doi: 10.1126/scisignal.2002413.

8.

Activated membrane patches guide chemotactic cell motility.

Hecht I, Skoge ML, Charest PG, Ben-Jacob E, Firtel RA, Loomis WF, Levine H, Rappel WJ.

PLoS Comput Biol. 2011 Jun;7(6):e1002044. doi: 10.1371/journal.pcbi.1002044. Epub 2011 Jun 30.

9.

"TORCing" neutrophil chemotaxis.

Charest PG, Firtel RA.

Dev Cell. 2010 Dec 14;19(6):795-6. doi: 10.1016/j.devcel.2010.11.017.

10.

A Ras signaling complex controls the RasC-TORC2 pathway and directed cell migration.

Charest PG, Shen Z, Lakoduk A, Sasaki AT, Briggs SP, Firtel RA.

Dev Cell. 2010 May 18;18(5):737-49. doi: 10.1016/j.devcel.2010.03.017.

11.

Spatiotemporal regulation of Ras activity provides directional sensing.

Zhang S, Charest PG, Firtel RA.

Curr Biol. 2008 Oct 28;18(20):1587-1593. doi: 10.1016/j.cub.2008.08.069.

12.

The regulation of cell motility and chemotaxis by phospholipid signaling.

Kölsch V, Charest PG, Firtel RA.

J Cell Sci. 2008 Mar 1;121(Pt 5):551-9. doi: 10.1242/jcs.023333. Review.

13.

Unraveling G protein-coupled receptor endocytosis pathways using real-time monitoring of agonist-promoted interaction between beta-arrestins and AP-2.

Hamdan FF, Rochdi MD, Breton B, Fessart D, Michaud DE, Charest PG, Laporte SA, Bouvier M.

J Biol Chem. 2007 Oct 5;282(40):29089-100. Epub 2007 Aug 3.

14.

G protein-independent Ras/PI3K/F-actin circuit regulates basic cell motility.

Sasaki AT, Janetopoulos C, Lee S, Charest PG, Takeda K, Sundheimer LW, Meili R, Devreotes PN, Firtel RA.

J Cell Biol. 2007 Jul 16;178(2):185-91.

15.

Big roles for small GTPases in the control of directed cell movement.

Charest PG, Firtel RA.

Biochem J. 2007 Jan 15;401(2):377-90. Review.

16.

The V2 vasopressin receptor stimulates ERK1/2 activity independently of heterotrimeric G protein signalling.

Charest PG, Oligny-Longpré G, Bonin H, Azzi M, Bouvier M.

Cell Signal. 2007 Jan;19(1):32-41. Epub 2006 Jun 3.

PMID:
16857342
17.

Feedback signaling controls leading-edge formation during chemotaxis.

Charest PG, Firtel RA.

Curr Opin Genet Dev. 2006 Aug;16(4):339-47. Epub 2006 Jun 27. Review.

PMID:
16806895
18.

Real-time monitoring of ubiquitination in living cells by BRET.

Perroy J, Pontier S, Charest PG, Aubry M, Bouvier M.

Nat Methods. 2004 Dec;1(3):203-8. Epub 2004 Nov 18.

PMID:
15782195
19.
20.

Beta-arrestin-mediated activation of MAPK by inverse agonists reveals distinct active conformations for G protein-coupled receptors.

Azzi M, Charest PG, Angers S, Rousseau G, Kohout T, Bouvier M, Piñeyro G.

Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11406-11. Epub 2003 Sep 17.

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