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Proc Natl Acad Sci U S A. 2017 Oct 24;114(43):11398-11403. doi: 10.1073/pnas.1705367114. Epub 2017 Oct 9.

PrimPol is required for replication reinitiation after mtDNA damage.

Author information

1
Department of Environmental and Biological Sciences, University of Eastern Finland, 80101 Joensuu, Finland.
2
Department of Medical Biochemistry and Biophysics, Umeå University, 901 87 Umeå, Sweden.
3
Centro de Biologia Molecular Severo Ochoa, E-28049 Madrid, Spain.
4
Department of Medical Biochemistry and Biophysics, Umeå University, 901 87 Umeå, Sweden; sjoerd.wanrooij@umu.se jaakko.pohjoismaki@uef.fi.
5
Department of Environmental and Biological Sciences, University of Eastern Finland, 80101 Joensuu, Finland; sjoerd.wanrooij@umu.se jaakko.pohjoismaki@uef.fi.

Abstract

Eukaryotic PrimPol is a recently discovered DNA-dependent DNA primase and translesion synthesis DNA polymerase found in the nucleus and mitochondria. Although PrimPol has been shown to be required for repriming of stalled replication forks in the nucleus, its role in mitochondria has remained unresolved. Here we demonstrate in vivo and in vitro that PrimPol can reinitiate stalled mtDNA replication and can prime mtDNA replication from nonconventional origins. Our results not only help in the understanding of how mitochondria cope with replicative stress but can also explain some controversial features of the lagging-strand replication.

KEYWORDS:

DNA repair; fork rescue; mtDNA damage; mtDNA replication

PMID:
29073063
PMCID:
PMC5664498
DOI:
10.1073/pnas.1705367114
[Indexed for MEDLINE]
Free PMC Article

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