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See 1 citation in 2018 by Arguijo-hernandez E:

Arch Virol. 2018 Nov;163(11):2959-2969. doi: 10.1007/s00705-018-3954-z. Epub 2018 Jul 24.

Cor interacts with outer membrane proteins to exclude FhuA-dependent phages.

Author information

1
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 07360, Mexico City (CDMX), México.
2
Unidad de Investigación Médica en Inmunología e Infectología, Centro Médico Nacional la Raza, IMSS, 02990, Mexico City (CDMX), México.
3
Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, 04510, Mexico City (CDMX), México.
4
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 07360, Mexico City (CDMX), México. luisk@cinvestav.mx.

Abstract

Superinfection exclusion (Sie) of FhuA-dependent phages is carried out by Cor in the Escherichia coli mEp167 prophage lysogenic strain. In this work, we present evidence that Cor is an outer membrane (OM) lipoprotein that requires the participation of additional outer membrane proteins (OMPs) to exclude FhuA-dependent phages. Two Cor species of ~13 and ~8.5 kDa, corresponding to the preprolipoprotein/prolipoprotein and lipoprotein, were observed by Western blot. Cell mutants for CorC17F, CorA18D and CorA57E lost the Sie phenotype for FhuA-dependent phages. A copurification affinity binding assay combined with LC_ESI_MS/MS showed that Cor bound to OMPs: OmpA, OmpC, OmpF, OmpW, LamB, and Slp. Interestingly, Sie for FhuA-dependent phages was reduced on Cor overexpressing FhuA+ mutant strains, where ompA, ompC, ompF, ompW, lamB, fhuE, genes were knocked out. The exclusion was restored when these strains were supplemented with plasmids expressing these genes. Sie was not lost in other Cor overexpressing FhuA+ null mutant strains JW3938(btuB-), JW5100(tolB-), JW3474(slp-). These results indicate that Cor interacts and requires some OMPs to exclude FhuA-dependent phages.

PMID:
30043202
DOI:
10.1007/s00705-018-3954-z
[Indexed for MEDLINE]

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