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Cell-free translation and purification of Arabidopsis thaliana regulator of G signaling 1 protein.

Li B, Makino SI, Beebe ET, Urano D, Aceti DJ, Misenheimer TM, Peters J, Fox BG, Jones AM.

Protein Expr Purif. 2016 Oct;126:33-41. doi: 10.1016/j.pep.2016.04.016. Epub 2016 May 6.


Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies.

Aceti DJ, Bingman CA, Wrobel RL, Frederick RO, Makino S, Nichols KW, Sahu SC, Bergeman LF, Blommel PG, Cornilescu CC, Gromek KA, Seder KD, Hwang S, Primm JG, Sabat G, Vojtik FC, Volkman BF, Zolnai Z, Phillips GN Jr, Markley JL, Fox BG.

J Struct Funct Genomics. 2015 Jun;16(2):67-80. doi: 10.1007/s10969-015-9198-1. Epub 2015 Apr 9.


Solution structure of the 2A protease from a common cold agent, human rhinovirus C2, strain W12.

Lee W, Watters KE, Troupis AT, Reinen NM, Suchy FP, Moyer KL, Frederick RO, Tonelli M, Aceti DJ, Palmenberg AC, Markley JL.

PLoS One. 2014 Jun 17;9(6):e97198. doi: 10.1371/journal.pone.0097198. eCollection 2014.


Structural and functional characterization of CalS11, a TDP-rhamnose 3'-O-methyltransferase involved in calicheamicin biosynthesis.

Singh S, Chang A, Helmich KE, Bingman CA, Wrobel RL, Beebe ET, Makino S, Aceti DJ, Dyer K, Hura GL, Sunkara M, Morris AJ, Phillips GN Jr, Thorson JS.

ACS Chem Biol. 2013 Jul 19;8(7):1632-9. doi: 10.1021/cb400068k. Epub 2013 May 23.


Mutations in FLS2 Ser-938 dissect signaling activation in FLS2-mediated Arabidopsis immunity.

Cao Y, Aceti DJ, Sabat G, Song J, Makino S, Fox BG, Bent AF.

PLoS Pathog. 2013;9(4):e1003313. doi: 10.1371/journal.ppat.1003313. Epub 2013 Apr 18.


The Center for Eukaryotic Structural Genomics.

Markley JL, Aceti DJ, Bingman CA, Fox BG, Frederick RO, Makino S, Nichols KW, Phillips GN Jr, Primm JG, Sahu SC, Vojtik FC, Volkman BF, Wrobel RL, Zolnai Z.

J Struct Funct Genomics. 2009 Apr;10(2):165-79. doi: 10.1007/s10969-008-9057-4. Epub 2009 Jan 8.


Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR.

Takeda M, Sugimori N, Torizawa T, Terauchi T, Ono AM, Yagi H, Yamaguchi Y, Kato K, Ikeya T, Jee J, G√ľntert P, Aceti DJ, Markley JL, Kainosho M.

FEBS J. 2008 Dec;275(23):5873-84. doi: 10.1111/j.1742-4658.2008.06717.x.


Structural and functional characterization of a novel phosphatase from the Arabidopsis thaliana gene locus At1g05000.

Aceti DJ, Bitto E, Yakunin AF, Proudfoot M, Bingman CA, Frederick RO, Sreenath HK, Vojtik FC, Wrobel RL, Fox BG, Markley JL, Phillips GN Jr.

Proteins. 2008 Oct;73(1):241-53. doi: 10.1002/prot.22041.


Small-scale, semi-automated purification of eukaryotic proteins for structure determination.

Frederick RO, Bergeman L, Blommel PG, Bailey LJ, McCoy JG, Song J, Meske L, Bingman CA, Riters M, Dillon NA, Kunert J, Yoon JW, Lim A, Cassidy M, Bunge J, Aceti DJ, Primm JG, Markley JL, Phillips GN Jr, Fox BG.

J Struct Funct Genomics. 2007 Dec;8(4):153-66. Epub 2007 Nov 6.


Crystal structure of Homo sapiens protein LOC79017.

Bae E, Bingman CA, Aceti DJ, Phillips GN Jr.

Proteins. 2008 Feb 1;70(2):588-91. No abstract available.


Crystal structure of Arabidopsis thaliana cytokinin dehydrogenase.

Bae E, Bingman CA, Bitto E, Aceti DJ, Phillips GN Jr.

Proteins. 2008 Jan 1;70(1):303-6. No abstract available.


The structure at 2.4 A resolution of the protein from gene locus At3g21360, a putative Fe(II)/2-oxoglutarate-dependent enzyme from Arabidopsis thaliana.

Bitto E, Bingman CA, Allard ST, Wesenberg GE, Aceti DJ, Wrobel RL, Frederick RO, Sreenath H, Vojtik FC, Jeon WB, Newman CS, Primm J, Sussman MR, Fox BG, Markley JL, Phillips GN Jr.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 May 1;61(Pt 5):469-72. Epub 2005 Apr 26.


Structure and mechanism of mouse cysteine dioxygenase.

McCoy JG, Bailey LJ, Bitto E, Bingman CA, Aceti DJ, Fox BG, Phillips GN Jr.

Proc Natl Acad Sci U S A. 2006 Feb 28;103(9):3084-9. Epub 2006 Feb 21.


High-throughput purification and quality assurance of Arabidopsis thaliana proteins for eukaryotic structural genomics.

Jeon WB, Aceti DJ, Bingman CA, Vojtik FC, Olson AC, Ellefson JM, McCombs JE, Sreenath HK, Blommel PG, Seder KD, Burns BT, Geetha HV, Harms AC, Sabat G, Sussman MR, Fox BG, Phillips GN Jr.

J Struct Funct Genomics. 2005;6(2-3):143-7.


Comparison of cell-based and cell-free protocols for producing target proteins from the Arabidopsis thaliana genome for structural studies.

Tyler RC, Aceti DJ, Bingman CA, Cornilescu CC, Fox BG, Frederick RO, Jeon WB, Lee MS, Newman CS, Peterson FC, Phillips GN Jr, Shahan MN, Singh S, Song J, Sreenath HK, Tyler EM, Ulrich EL, Vinarov DA, Vojtik FC, Volkman BF, Wrobel RL, Zhao Q, Markley JL.

Proteins. 2005 May 15;59(3):633-43.


Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination.

Tyler RC, Sreenath HK, Singh S, Aceti DJ, Bingman CA, Markley JL, Fox BG.

Protein Expr Purif. 2005 Apr;40(2):268-78.


Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium.

Sreenath HK, Bingman CA, Buchan BW, Seder KD, Burns BT, Geetha HV, Jeon WB, Vojtik FC, Aceti DJ, Frederick RO, Phillips GN Jr, Fox BG.

Protein Expr Purif. 2005 Apr;40(2):256-67.


(1)H, (13)C and (15)N backbone assignment of a 32 kDa hypothetical protein from Arabidopsis thaliana, At3g16450.1.

Sugimori N, Torizawa T, Aceti DJ, Thao S, Markley JL, Kainosho M.

J Biomol NMR. 2004 Nov;30(3):357-8. No abstract available.


Crystal structure of At2g03760, a putative steroid sulfotransferase from Arabidopsis thaliana.

Smith DW, Johnson KA, Bingman CA, Aceti DJ, Blommel PG, Wrobel RL, Frederick RO, Zhao Q, Sreenath H, Fox BG, Volkman BF, Jeon WB, Newman CS, Ulrich EL, Hegeman AD, Kimball T, Thao S, Sussman MR, Markley JL, Phillips GN Jr.

Proteins. 2004 Dec 1;57(4):854-7. No abstract available.


Critical regions for the sweetness of brazzein.

Jin Z, Danilova V, Assadi-Porter FM, Aceti DJ, Markley JL, Hellekant G.

FEBS Lett. 2003 Jun 5;544(1-3):33-7.

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