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Proc Natl Acad Sci U S A. 2016 Oct 25;113(43):E6582-E6589. Epub 2016 Oct 11.

Working stroke of the kinesin-14, ncd, comprises two substeps of different direction.

Author information

  • 1Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany; Center for Molecular Bioengineering (B CUBE), Technische Universit├Ąt Dresden, 01069 Dresden, Germany.
  • 2Nencki Institute of Experimental Biology, 02-093 Warsaw, Poland.
  • 3J. Stefan Institute, 1000 Ljubljana, Slovenia andrej.vilfan@ijs.si diez@bcube-dresden.de.
  • 4Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany; Center for Molecular Bioengineering (B CUBE), Technische Universit├Ąt Dresden, 01069 Dresden, Germany; andrej.vilfan@ijs.si diez@bcube-dresden.de.

Abstract

Single-molecule experiments have been used with great success to explore the mechanochemical cycles of processive motor proteins such as kinesin-1, but it has proven difficult to apply these approaches to nonprocessive motors. Therefore, the mechanochemical cycle of kinesin-14 (ncd) is still under debate. Here, we use the readout from the collective activity of multiple motors to derive information about the mechanochemical cycle of individual ncd motors. In gliding motility assays we performed 3D imaging based on fluorescence interference contrast microscopy combined with nanometer tracking to simultaneously study the translation and rotation of microtubules. Microtubules gliding on ncd-coated surfaces rotated around their longitudinal axes in an [ATP]- and [ADP]-dependent manner. Combined with a simple mechanical model, these observations suggest that the working stroke of ncd consists of an initial small movement of its stalk in a lateral direction when ADP is released and a second, main component of the working stroke, in a longitudinal direction upon ATP binding.

KEYWORDS:

fluorescence interference contrast microscopy; gliding motility assay; kinesin; mathematical modeling; microtubule

PMID:
27729532
PMCID:
PMC5086986
[Available on 2017-04-25]
DOI:
10.1073/pnas.1525313113
[PubMed - in process]
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