Abstract
Defatted sesame seeds have been reported for hypoglycemic effect in mice and T2DM women. An attempted to identify active components responsible for this effect was conducted using α-glucosidase-guided fractionation, resulting in the isolation of various lignans. Of compounds isolated, only (+)-pinoresinol showed inhibitory activity against rat intestinal maltase with an IC(50) value of 34.3 μM. The kinetic study indicated that enzymatic hydrolysis of maltose is inhibited by (+)-pinoresinol through competitive and noncompetitive manners. However, a lower dissociation constant (k(i) 288 M) of EI complex suggested that competitive inhibition is predominant over noncompetitive mode (k'(i) 1342 M).
Copyright © 2012 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Diabetes Mellitus, Type 2 / drug therapy
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Enzyme Inhibitors / chemistry*
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Enzyme Inhibitors / isolation & purification
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Enzyme Inhibitors / therapeutic use
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Female
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Furans / chemistry*
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Furans / isolation & purification
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Furans / therapeutic use
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Glycoside Hydrolase Inhibitors*
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Humans
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Hypoglycemic Agents / chemistry*
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Hypoglycemic Agents / isolation & purification
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Hypoglycemic Agents / therapeutic use
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Intestines / enzymology
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Kinetics
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Lignans / chemistry*
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Lignans / isolation & purification
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Lignans / therapeutic use
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Mice
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Rats
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Seeds / chemistry
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Sesamum / chemistry*
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Stereoisomerism
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alpha-Glucosidases / metabolism
Substances
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Enzyme Inhibitors
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Furans
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Glycoside Hydrolase Inhibitors
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Hypoglycemic Agents
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Lignans
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alpha-Glucosidases
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pinoresinol