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Toxicol Sci. 2016 Jun;151(2):312-23. doi: 10.1093/toxsci/kfw045. Epub 2016 Mar 8.

Vinyl Chloride Metabolites Potentiate Inflammatory Liver Injury Caused by LPS in Mice.

Author information

1
*Department of Pharmacology and Toxicology Department of Medicine.
2
*Department of Pharmacology and Toxicology.
3
Department of Medicine.
4
Department of Medicine Diabetes and Obesity Center.
5
Department of Microbiology and Immunology, University of Louisville Health Sciences Center, Louisville, Kentucky 40292; and.
6
*Department of Pharmacology and Toxicology Department of Medicine Robley Rex Louisville VAMC, Louisville, Kentucky 40206.
7
*Department of Pharmacology and Toxicology juliane.beier@louisville.edu.

Abstract

Vinyl chloride (VC) is a ubiquitous environmental contaminant for which human risk is incompletely understood. We have previously reported that high occupational exposure to VC directly caused liver damage in humans. However, whether VC may also potentiate liver injury from other causes is not known. C57Bl/6J mice were administered chloroethanol (CE), a major metabolite of VC, and lipopolysaccharide (LPS) 24 h after CE. Samples were harvested for determination of liver damage, inflammation, and changes in carbohydrate and lipid metabolism. In mice, CE exposure alone caused no detectable liver damage. LPS exposure caused inflammatory liver damage, oxidative stress, lipid accumulation, and glycogen depletion; the effect of all of these variables was potentiated by CE pre-exposure. In vitro experiments suggest that VC metabolite chloroacetaldehyde (CAA) directly damages mitochondria, which may explain the sensitization effect observed in vivo Moreover, co-exposure of cells to CAA and TNFα caused increased cell death, supporting the hypothesis of sensitization by VC metabolites. Taken together, these data demonstrate that exposure to VC/metabolites at levels that are not overtly hepatotoxic can potentiate liver injury caused by another hepatotoxicant. This serves as proof-of-concept that VC hepatotoxicity may be modified by an additional metabolic stress such as endotoxemia, which commonly occurs in acute (eg, sepsis) and chronic (eg, NAFLD) diseases.

KEYWORDS:

PVC; TASH; hepatotoxicity.; toxicant-associated steatohepatitis; vinyl chloride metabolite

PMID:
26962056
PMCID:
PMC4880135
DOI:
10.1093/toxsci/kfw045
[Indexed for MEDLINE]
Free PMC Article

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