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Head Neck. 2007 Sep;29(9):803-14.

Tumor-specific genetic expression profile of metastatic oral squamous cell carcinoma.

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1
Department of Otolaryngology - Head and Neck Surgery, University of Washington, Seattle, Washington 98195, USA.

Abstract

BACKGROUND:

Metastasis is the most important predictor of survival in patients with oral squamous cell carcinoma (OSCC). We tested the hypothesis that there is a genetic expression profile associated with OSCC metastasis.

METHODS:

We obtained samples from 6 OSCC node-positive primary tumors and their matched metastatic lymph nodes, and 5 OSCC node-negative primary tumors. Using laser capture microdissection, we isolated OSCC cells from metastatic lymph nodes and compared them with those from matched primary tumors and unmatched node-negative primary tumors using Affymetrix Human Genome Focus arrays.

RESULTS:

Comparison of tumor cells from the lymph nodes with those from the unmatched, node-negative primary tumors revealed differential expression of 160 genes. Hierarchical clustering and principal component analysis using this 160-gene set showed that the node-negative samples were distinguishable from both, node-positive primary tumors and tumors in the lymph nodes. Many of the expression changes found in the metastatic cells from the lymph nodes were also found in the node-positive primary tumors. Immunohistochemical analysis for transglutaminase-3 and keratin 16 confirmed the differential genetic expression for these genes.

CONCLUSION:

These preliminary results suggest that there may be a metastatic gene expression profile present in node-positive primary OSCC.

PMID:
17573689
DOI:
10.1002/hed.20598
[Indexed for MEDLINE]
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