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The air-liquid interface and use of primary cell cultures are important to recapitulate the transcriptional profile of in vivo airway epithelia.
Pezzulo AA, Starner TD, Scheetz TE, Traver GL, Tilley AE, Harvey BG, Crystal RG, McCray PB Jr, Zabner J. Pezzulo AA, et al. Among authors: traver gl. Am J Physiol Lung Cell Mol Physiol. 2011 Jan;300(1):L25-31. doi: 10.1152/ajplung.00256.2010. Epub 2010 Oct 22. Am J Physiol Lung Cell Mol Physiol. 2011. PMID: 20971803 Free PMC article.