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Reprod Toxicol. 2015 Jan;51:106-13. doi: 10.1016/j.reprotox.2015.01.001. Epub 2015 Jan 25.

Transferability and inter-laboratory variability assessment of the in vitro bovine oocyte fertilization test.

Author information

1
Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety, University of Milan, 20133 Milan, Italy.
2
Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety, University of Milan, 20133 Milan, Italy; Interdepartmental Research Centre for the Study of Biological Effects of Nano-Concentrations (CREBION), University of Milan, 20133 Milan, Italy.
3
Avantea, Laboratory of Reproductive Technologies, 26100 Cremona, Italy.
4
Avantea, Laboratory of Reproductive Technologies, 26100 Cremona, Italy; Dipartimento di Scienze Mediche Veterinarie, Università di Bologna, 40064 Bologna, Italy; Fondazione Avantea, Cremona, Italy.
5
Avantea, Laboratory of Reproductive Technologies, 26100 Cremona, Italy; Fondazione Avantea, Cremona, Italy.
6
Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety, University of Milan, 20133 Milan, Italy; Interdepartmental Research Centre for the Study of Biological Effects of Nano-Concentrations (CREBION), University of Milan, 20133 Milan, Italy. Electronic address: alberto.luciano@unimi.it.

Abstract

The dramatic increase in the number of animals required for reproductive toxicity testing imposes the validation of alternative methods to reduce the use of laboratory animals. As we previously demonstrated for in vitro maturation test of bovine oocytes, the present study describes the transferability assessment and the inter-laboratory variability of an in vitro test able to identify chemical effects during the process of bovine oocyte fertilization. Eight chemicals with well-known toxic properties (benzo[a]pyrene, busulfan, cadmium chloride, cycloheximide, diethylstilbestrol, ketoconazole, methylacetoacetate, mifepristone/RU-486) were tested in two well-trained laboratories. The statistical analysis demonstrated no differences in the EC50 values for each chemical in within (inter-runs) and in between-laboratory variability of the proposed test. We therefore conclude that the bovine in vitro fertilization test could advance toward the validation process as alternative in vitro method and become part of an integrated testing strategy in order to predict chemical hazards on mammalian fertility.

KEYWORDS:

3Rs principle; Alternative methods; Bovine; Fertilization; Oocyte; ReProTect; Reproductive toxicity; Spermatozoa

PMID:
25625651
DOI:
10.1016/j.reprotox.2015.01.001
[Indexed for MEDLINE]

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