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J Insect Sci. 2015 Jun 25;15. pii: 79. doi: 10.1093/jisesa/iev065. Print 2015.

Transcription Analysis of the Beta-Glucosidase Precursor in Wild-Type and l-4i Mutant Bombyx mori (Lepidoptera: Bombycidae).

Author information

1
School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China.
2
BGI-Shenzhen, Shenzhen 518083, China.
3
School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China The Sericulture Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang Jiangsu 212018, China qlzhao302@126.com.

Abstract

Lethal fourth-instar larvae (l-4i) mutant of Bombyx mori, a recently discovered novel mutant, die from energy depletion due to genetic mutation. Beta-glucosidase is a common digestive enzyme that hydrolyzes cellulose in the diet to provide energy. In this study, the mRNA expression profiles of B. mori beta-glucosidase precursor (BmpreBG) were characterized by reverse transcription polymerase chain reaction and quantitative real-time polymerase chain reaction. The transcription level of BmpreBG varied in different tissues and developmental stages, except in the pupa and moth, which are the no-diet period. Remarkably, the mRNA expression level of BmpreBG was sharply reduced in l-4i but not in the wild type, which suggested that the digestive function of the mutant was severely damaged. This was consistent with the l-4i phenotypic traits of not eating mulberries, lack of energy, and ultimate death. 5'-rapid amplification of cDNA ends showed, for the first time, that BmpreBG has a 160-bp 5'-untranslated region. These findings suggested that B. mori β-glucosidase precursor was involved in the death process of l-4i mutant larvae.

KEYWORDS:

BmpreBG; lethal mutant; β-glucosidase precursor

PMID:
26113511
DOI:
10.1093/jisesa/iev065
[Indexed for MEDLINE]

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