Send to

Choose Destination
  • The following term was not found in PubMed: andl-4i.
J Insect Sci. 2015 Jun 25;15. pii: 79. doi: 10.1093/jisesa/iev065. Print 2015.

Transcription Analysis of the Beta-Glucosidase Precursor in Wild-Type and l-4i Mutant Bombyx mori (Lepidoptera: Bombycidae).

Author information

School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China.
BGI-Shenzhen, Shenzhen 518083, China.
School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China The Sericulture Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang Jiangsu 212018, China


Lethal fourth-instar larvae (l-4i) mutant of Bombyx mori, a recently discovered novel mutant, die from energy depletion due to genetic mutation. Beta-glucosidase is a common digestive enzyme that hydrolyzes cellulose in the diet to provide energy. In this study, the mRNA expression profiles of B. mori beta-glucosidase precursor (BmpreBG) were characterized by reverse transcription polymerase chain reaction and quantitative real-time polymerase chain reaction. The transcription level of BmpreBG varied in different tissues and developmental stages, except in the pupa and moth, which are the no-diet period. Remarkably, the mRNA expression level of BmpreBG was sharply reduced in l-4i but not in the wild type, which suggested that the digestive function of the mutant was severely damaged. This was consistent with the l-4i phenotypic traits of not eating mulberries, lack of energy, and ultimate death. 5'-rapid amplification of cDNA ends showed, for the first time, that BmpreBG has a 160-bp 5'-untranslated region. These findings suggested that B. mori β-glucosidase precursor was involved in the death process of l-4i mutant larvae.


BmpreBG; lethal mutant; β-glucosidase precursor

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center