Format

Send to

Choose Destination

See 1 citation found by title matching your search:

J Control Release. 2018 Apr 10;275:40-52. doi: 10.1016/j.jconrel.2018.02.004. Epub 2018 Feb 8.

The use of gold nanorods as a new vaccine platform against schistosomiasis.

Author information

1
Laboratório de Imunologia de Doenças Infecciosas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil; Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT-DT), Conselho Nacional de Desenvolvimento Científico e Tecnológico, Ministério de Ciência Tecnologia e Inovação Salvador, Bahia, Brazil.
2
Center of Nanoscience, Nanotechnology and Innovation - CeNano2I, Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, Brazil.
3
Laboratório de Nanomateriais, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
4
Laboratório de Imunologia de Doenças Infecciosas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil; Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT-DT), Conselho Nacional de Desenvolvimento Científico e Tecnológico, Ministério de Ciência Tecnologia e Inovação Salvador, Bahia, Brazil. Electronic address: scozeus@icb.ufmg.br.

Abstract

Schistosomiasis is an important parasitic disease affecting >207 million people in 76 countries around the world and causing approximately 250,000 deaths per year. At present, the main strategy adopted for the control of schistosomiasis is the use of safe chemotherapy, such as praziquantel. However, the high rates of reinfection after treatment restrict the use of this treatment approach and assume the need for other forms of control such as vaccination. Sm29 is a protein that is localized in the Schistosoma mansoni tegument of adult worms and schistosomula and is considered a powerful vaccine candidate. Because of the chemical, physical and immunological characteristics of nanoparticles, nanocarriers have received increasing attention. In the field of nanotechnology, gold nanorods are considered potential vaccine carriers. In this study, we bound S. mansoni rSm29 protein to gold nanorods either directly or by cysteamine functionalization. When the worm burden was evaluated, the AuNRs-NH2-rSm29 group of immunized mice showed the best protection level (34%). Following AuNRs-NH2-rSm29 immunization, we observed a Th1 immunological response in mice with higher production of IFN-γ, mainly by CD4+ and CD8+ T cells. Furthermore, AuNRs-NH2-rSm29 could activate dendritic cells in vitro, enhancing MHCII and MHCI expression and the production of IL-1β in a NLRP3-, ASC- and Caspase-1-dependent manner. In summary, our findings support the use of nanorods as an immunization strategy in vaccine development against infectious diseases.

KEYWORDS:

Gold nanorods; Inflammasome; NLRP3; Nanocarriers; Recombinant vaccine; S. mansoni

PMID:
29428201
DOI:
10.1016/j.jconrel.2018.02.004
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center