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Pathog Dis. 2014 Jul;71(2):164-76. doi: 10.1111/2049-632X.12136. Epub 2014 Mar 20.

The use of Nanotrap particles for biodefense and emerging infectious disease diagnostics.

Author information

1
National Center for Biodefense and Infectious Diseases, School of Systems Biology, George Mason University, Manassas, VA, USA.

Abstract

Detection of early infectious disease may be challenging due to the low copy number of organisms present. To overcome this limitation and rapidly measure low concentrations of the pathogen, we developed a novel technology: Nanotrap particles, which are designed to capture, concentrate, and protect biomarkers from complex biofluids. Nanotrap particles are thermoresponsive hydrogels that are capable of antigen capture through the coupling of affinity baits to the particles. Here, we describe recent findings demonstrating that Nanotrap particles are able to capture live infectious virus, viral RNA, and viral proteins. Capture is possible even in complex mixtures such as serum and allows the concentration and protection of these analytes, providing increased performance of downstream assays. The Nanotrap particles are a versatile sample preparation technology that has far reaching implications for biomarker discovery and diagnostic assays.

KEYWORDS:

Rift Valley fever virus; Venezuelan equine encephalitis virus; diagnostics; influenza

PMID:
24449537
DOI:
10.1111/2049-632X.12136
[Indexed for MEDLINE]

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