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Nat Commun. 2019 Jun 28;10(1):2856. doi: 10.1038/s41467-019-10779-9.

The structure of a 15-stranded actin-like filament from Clostridium botulinum.

Author information

1
Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore, 138673, Singapore.
2
Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, 138632, Singapore.
3
Structural Biology Research Center, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan. narita.akihiro@f.mbox.nagoya-u.ac.jp.
4
Structural Biology Research Center, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan.
5
Department of Physics and Information Technology, Graduate School of Computer Science and Systems Engineering, Kyushu Institute of Technology, Kawazu 680-4, Iizuka, Fukuoka, 820-8502, Japan.
6
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, 10032, USA.
7
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, 10027, USA.
8
Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore, 138673, Singapore. thebamboorodmaker@gmail.com.
9
Fly&Fish Lab, Ebino, Miyazaki, 889-4163, Japan. thebamboorodmaker@gmail.com.
10
School of Biomolecular Science and Engineering (BSE), Vidyasirimedhi Institute of Science and Technology (VISTEC), Rayong, 21210, Thailand.
11
Research Institute for Interdisciplinary Science, Okayama University, Okayama, 700-8530, Japan.

Abstract

Microfilaments (actin) and microtubules represent the extremes in eukaryotic cytoskeleton cross-sectional dimensions, raising the question of whether filament architectures are limited by protein fold. Here, we report the cryoelectron microscopy structure of a complex filament formed from 15 protofilaments of an actin-like protein. This actin-like ParM is encoded on the large pCBH Clostridium botulinum plasmid. In cross-section, the ~26 nm diameter filament comprises a central helical protofilament surrounded by intermediate and outer layers of six and eight twisted protofilaments, respectively. Alternating polarity of the layers allows for similar lateral contacts between each layer. This filament design is stiffer than the actin filament, and has likely been selected for during evolution to move large cargos. The comparable sizes of microtubule and pCBH ParM filaments indicate that larger filament architectures are not limited by the protomer fold. Instead, function appears to have been the evolutionary driving force to produce broad, complex filaments.

PMID:
31253774
PMCID:
PMC6599009
DOI:
10.1038/s41467-019-10779-9
[Indexed for MEDLINE]
Free PMC Article

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