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Eur J Protistol. 2019 Jun;69:14-19. doi: 10.1016/j.ejop.2019.02.004. Epub 2019 Feb 14.

The opportunistic pathogen Encephalitozoon cuniculi in wild living Murinae and Arvicolinae in Central Europe.

Author information

1
Department of Parasitology, Institute of Genetics and Microbiology, University of Wrocław, Przybyszewskiego 63, 51-148 Wrocław, Poland. Electronic address: agnieszka.perec-matysiak@uwr.edu.pl.
2
Department of Parasitology, Institute of Genetics and Microbiology, University of Wrocław, Przybyszewskiego 63, 51-148 Wrocław, Poland.
3
Faculty of Agriculture, University of South Bohemia in České Budějovice, Studentská 13, 370 05 České Budějovice, Czech Republic; Institute of Parasitology, the Biology Centre of the Czech Academy of Sciences, Branišovská 31, 370 05 České Budějovice, Czech Republic.
4
Institute of Parasitology, the Biology Centre of the Czech Academy of Sciences, Branišovská 31, 370 05 České Budějovice, Czech Republic.
5
Faculty of Forestry, Department of Forest Protection and Wildlife Management, Technical University in Zvolen, Zvolen, Slovak Republic.

Abstract

Encephalitozoon spp. is an obligate intracellular microsporidian parasite that infects a wide range of mammalian hosts, including humans. This study was conducted to determine the prevalence of Encephalitozoon spp. in wild living rodents from Poland, the Czech Republic and Slovakia. Faecal and spleen samples were collected from individuals of Apodemus agrarius, Apodemus flavicollis, Apodemus sylvaticus, and Myodes glareolus (n = 465) and used for DNA extraction. PCR, targeting the ITS region of the rRNA gene was performed. The overall prevalence of microsporidia was 15.1%. The occurrence of Encephalitozoon cuniculi in the abovementioned host species of rodents has been presented for the first time, with the highest infection rate recorded for A. flavicollis. Sequence analysis showed that the most frequent species was E. cuniculi genotype II (92.5%). E. cuniculi genotypes I (1.5%) and III (6.0%) were also identified.

KEYWORDS:

Encephalitozoon cuniculi; Microsporidia; Molecular analysis; Small rodents

PMID:
30825553
DOI:
10.1016/j.ejop.2019.02.004
[Indexed for MEDLINE]

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