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The involvement of the nuclear lamina in human and rodent spermiogenesis: a systematic review.
- 1
- 1Aix Marseille Univ, Inserm, MMG, U1251, Marseille Medical Genetics, 13385 Marseille, France.
- 2
- APHM Hôpital La Conception, Pôle femmes-Parents-enfants, Centre Clinico-Biologique d'Assistance Médicale à la Procréation-CECOS, 13385 Marseille Cedex 5, France.
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- 3Genetic Epigenetic and Therapies of Infertility, Institute for Advanced Biosciences, Inserm U1209, CNRS UMR 5309, Université Grenoble Alpes, CHU Grenoble Alpes, F-38000 Grenoble, France.
Abstract
in
English,
FrenchLa lamina nucléaire (LN) est un réseau de filaments protéiques, composé essentiellement de lamines, situé entre la membrane nucléaire interne et la chromatine. La LN est un composant de l’enveloppe nucléaire, interagit avec une large gamme de protéines et est nécessaire à l’intégrité de la structure nucléaire et au développement physiologique. Au cours de la spermiogenèse, le noyau de la spermatide s’allonge et sa taille est considérablement réduite, les protamines remplaçant les histones dans le but de constituer une chromatine fortement compactée. De nombreux travaux chez l’homme et chez les rongeurs montrent que la LN joue un rôle important dans la différenciation des spermatides chez les mammifères au cours de la spermiogenèse. Dans cette revue, nous résumons et discutons les données disponibles dans la littérature concernant l’implication des lamines et de leurs partenaires directs ou indirects dans la spermiogenèse humaine normale et anormale.
The nuclear lamina (NL) is a filamentous protein meshwork, composed essentially of lamins, situated between the inner nuclear membrane and the chromatin. The NL is a component of the nuclear envelope, interacts with a wide range of proteins and is required for normal nuclear structure and physiological development. During spermiogenesis the spermatid nucleus is elongated, and dramatically reduced in size with protamines replacing histones to produce a highly compacted chromatin. There is mounting evidence from studies in human and rodent, that the NL plays an important role in mammalian spermatid differentiation during spermiogenesis. In this review, we summarize and discuss the data available in the literature regarding the involvement of lamins and their direct or indirect partners in normal and abnormal human spermiogenesis.
KEYWORDS:
Chromatin; Human; Lamin; Nuclear lamina; Remodelling; Spermiogenesis
Fig. 1
a Immunolocalisation of Lamin B1, LEMD1, BAF and BAF-L (green) on human spermatids and testicular spermatozoa. Labelling is shown on successive steps of spermiogenesis: round spermatid (RS), elongating spermatid (ES), testicular spermatozoa (TS). The acrosome is identified using lectin PNA (red) and DNA is counterstained with DAPI (blue). Scale bar is 10 μm. b Schematic representation of nuclear lamina proteins and nuclear partner proteins during human spermiogenesis. Successive steps are represented: round spermatid (RS), elongating spermatid (ES), testicular spermatozoa (TS). The acrosome is represented in green. LEMD1: LEM domain containing 1; LEMD2-Cter: LEM domain containing 2-Cterminal region; BAF: Barrier-to-Autointegration Factor; BAF-L: Barrier-to-Autointegration Factor Like; LAP2b: LAP2: lamina-associated polypeptide 2-β isoform
Basic Clin Androl. 2018;28:7.
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