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Genes Dev. 2016 Jun 1;30(11):1313-26. doi: 10.1101/gad.275073.115. Epub 2016 Jun 9.

The CENP-T/-W complex is a binding partner of the histone chaperone FACT.

Author information

1
UMR3664, Centre National de la Recherche Scientifique, Institut Curie, PSL (Paris Sciences et Lettres) Research University, F-75005 Paris, France; UMR3664, Centre National de la Recherche Scientifique, University Pierre and Marie Curie Paris 06, Sorbonne Universités, F-75005 Paris, France;
2
Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10065, USA;
3
UMR3664, Centre National de la Recherche Scientifique, Institut Curie, PSL (Paris Sciences et Lettres) Research University, F-75005 Paris, France; Laboratoire de Spectrométrie de Masse Protéomique, Institut Curie, PSL (Paris Sciences et Lettres) Research University Centre de Recherche, Paris 75005, France;
4
Centre for Chromosome Biology, School of Natural Sciences, National University of Ireland, Galway, Ireland.

Abstract

The CENP-T/-W histone fold complex, as an integral part of the inner kinetochore, is essential for building a proper kinetochore at the centromere in order to direct chromosome segregation during mitosis. Notably, CENP-T/-W is not inherited at centromeres, and new deposition is absolutely required at each cell cycle for kinetochore function. However, the mechanisms underlying this new deposition of CENP-T/-W at centromeres are unclear. Here, we found that CENP-T deposition at centromeres is uncoupled from DNA synthesis. We identified Spt16 and SSRP1, subunits of the H2A-H2B histone chaperone facilitates chromatin transcription (FACT), as CENP-W binding partners through a proteomic screen. We found that the C-terminal region of Spt16 binds specifically to the histone fold region of CENP-T/-W. Furthermore, depletion of Spt16 impairs CENP-T and CENP-W deposition at endogenous centromeres, and site-directed targeting of Spt16 alone is sufficient to ensure local de novo CENP-T accumulation. We propose a model in which the FACT chaperone stabilizes the soluble CENP-T/-W complex in the cell and promotes dynamics of exchange, enabling CENP-T/-W deposition at centromeres.

KEYWORDS:

CENP; centromere; histone chaperone; mitosis

Comment in

PMID:
27284163
PMCID:
PMC4911930
DOI:
10.1101/gad.275073.115
[Indexed for MEDLINE]
Free PMC Article

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