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Items: 1 to 20 of 160


Effect of trehalose on the preservation of freeze-dried mice spermatozoa at room temperature.

Ito D, Wakayama S, Kamada Y, Shibasaki I, Kamimura S, Ooga M, Wakayama T.

J Reprod Dev. 2019 Aug 9;65(4):353-359. doi: 10.1262/jrd.2019-058. Epub 2019 May 23.


Zfp281 Shapes the Transcriptome of Trophoblast Stem Cells and Is Essential for Placental Development.

Ishiuchi T, Ohishi H, Sato T, Kamimura S, Yorino M, Abe S, Suzuki A, Wakayama T, Suyama M, Sasaki H.

Cell Rep. 2019 May 7;27(6):1742-1754.e6. doi: 10.1016/j.celrep.2019.04.028.


Tolerance of the freeze-dried mouse sperm nucleus to temperatures ranging from -196 °C to 150 °C.

Wakayama S, Ito D, Kamada Y, Yonemura S, Ooga M, Kishigami S, Wakayama T.

Sci Rep. 2019 Apr 5;9(1):5719. doi: 10.1038/s41598-019-42062-8.


Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection.

Wakayama S, Kishigami S, Wakayama T.

Methods Mol Biol. 2019;1874:211-228. doi: 10.1007/978-1-4939-8831-0_12.


Generation of two-cell cloned embryos from mouse faecal cell.

Kamimura S, Wakayama S, Kuwayama H, Tanabe Y, Kishigami S, Wakayama T.

Sci Rep. 2018 Oct 8;8(1):14922. doi: 10.1038/s41598-018-33304-2.


Single nucleolus precursor body formation in the pronucleus of mouse zygotes and SCNT embryos.

Kyogoku H, Wakayama T, Kitajima TS, Miyano T.

PLoS One. 2018 Aug 20;13(8):e0202663. doi: 10.1371/journal.pone.0202663. eCollection 2018.


Assessing the tolerance to room temperature and viability of freeze-dried mice spermatozoa over long-term storage at room temperature under vacuum.

Kamada Y, Wakayama S, Shibasaki I, Ito D, Kamimura S, Ooga M, Wakayama T.

Sci Rep. 2018 Jul 13;8(1):10602. doi: 10.1038/s41598-018-28896-8.


Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development.

Ooga M, Funaya S, Aoki F, Wakayama T.

J Vis Exp. 2018 Jun 12;(136). doi: 10.3791/57068.


Agarose capsules as new tools for protecting denuded mouse oocytes/embryos during handling and freezing-thawing and supporting embryonic development in vivo.

Nagatomo H, Yao T, Araki Y, Mizutani E, Wakayama T.

Sci Rep. 2017 Dec 20;7(1):17960. doi: 10.1038/s41598-017-18365-z.


Production of cloned mice using oocytes derived from ICR-outbred strain.

Tanabe Y, Kuwayama H, Wakayama S, Nagatomo H, Ooga M, Kamimura S, Kishigami S, Wakayama T.

Reproduction. 2017 Dec;154(6):859-866. doi: 10.1530/REP-17-0372. Epub 2017 Oct 2.


Reply to Ferlazzo and Foray: About the Space Pup project.

Wakayama S, Kamada Y, Yamanaka K, Kohda T, Suzuki H, Shimazu T, Tada MN, Osada I, Nagamatsu A, Kamimura S, Nagatomo H, Mizutani E, Ishino F, Yano S, Wakayama T.

Proc Natl Acad Sci U S A. 2017 Aug 15;114(33):E6734. doi: 10.1073/pnas.1711468114. Epub 2017 Aug 1. No abstract available.


Derivation of ground-state female ES cells maintaining gamete-derived DNA methylation.

Yagi M, Kishigami S, Tanaka A, Semi K, Mizutani E, Wakayama S, Wakayama T, Yamamoto T, Yamada Y.

Nature. 2017 Aug 10;548(7666):224-227. doi: 10.1038/nature23286. Epub 2017 Jul 26.


Reconstitution of the oocyte nucleolus in mice through a single nucleolar protein, NPM2.

Ogushi S, Yamagata K, Obuse C, Furuta K, Wakayama T, Matzuk MM, Saitou M.

J Cell Sci. 2017 Jul 15;130(14):2416-2429. doi: 10.1242/jcs.195875. Epub 2017 Jun 9.


FRAP analysis of chromatin looseness in mouse zygotes that allows full-term development.

Ooga M, Wakayama T.

PLoS One. 2017 May 22;12(5):e0178255. doi: 10.1371/journal.pone.0178255. eCollection 2017.


Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months.

Wakayama S, Kamada Y, Yamanaka K, Kohda T, Suzuki H, Shimazu T, Tada MN, Osada I, Nagamatsu A, Kamimura S, Nagatomo H, Mizutani E, Ishino F, Yano S, Wakayama T.

Proc Natl Acad Sci U S A. 2017 Jun 6;114(23):5988-5993. doi: 10.1073/pnas.1701425114. Epub 2017 May 22.


Birth of cloned mice from vaginal smear cells after somatic cell nuclear transfer.

Kuwayama H, Tanabe Y, Wakayama T, Kishigami S.

Theriogenology. 2017 May;94:79-85. doi: 10.1016/j.theriogenology.2017.02.012. Epub 2017 Feb 20.


The Number of Point Mutations in Induced Pluripotent Stem Cells and Nuclear Transfer Embryonic Stem Cells Depends on the Method and Somatic Cell Type Used for Their Generation.

Araki R, Mizutani E, Hoki Y, Sunayama M, Wakayama S, Nagatomo H, Kasama Y, Nakamura M, Wakayama T, Abe M.

Stem Cells. 2017 May;35(5):1189-1196. doi: 10.1002/stem.2601. Epub 2017 Mar 27.


Aberrant Expression of TIMP-2 and PBEF Genes in the Placentae of Cloned Mice Due to Epigenetic Reprogramming Error.

Kim HR, Lee JE, Oqani RK, Kim SY, Wakayama T, Li C, Sa SJ, Woo JS, Jin DI.

PLoS One. 2016 Nov 17;11(11):e0166241. doi: 10.1371/journal.pone.0166241. eCollection 2016.


Effect of Long-Term Exposure of Donor Nuclei to the Oocyte Cytoplasm on Production of Cloned Mice Using Serial Nuclear Transfer.

Wakayama S, Tanabe Y, Nagatomo H, Mizutani E, Kishigami S, Wakayama T.

Cell Reprogram. 2016 Nov;18(6):382-389. Epub 2016 Sep 13.


Phenotypes of Aging Postovulatory Oocytes After Somatic Cell Nuclear Transfer in Mice.

Lee AR, Shimoike T, Wakayama T, Kishigami S.

Cell Reprogram. 2016 Jun;18(3):147-53. doi: 10.1089/cell.2016.0014.


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