Drosophila hold'em is required for a subset of meiotic crossovers and interacts with the dna repair endonuclease complex subunits MEI-9 and ERCC1

Genetics. 2009 Jan;181(1):335-40. doi: 10.1534/genetics.108.093104. Epub 2008 Oct 28.

Abstract

Three Drosophila proteins, ERCC1, MUS312, and MEI-9, function in a complex proposed to resolve double-Holliday-junction intermediates into crossovers during meiosis. We report here the characterization of hold'em (hdm), whose protein product belongs to a single-strand-DNA-binding superfamily of proteins. Mutations in hdm result in reduced meiotic crossover formation and sensitivity to the DNA-damaging agent methyl methanesulfonate. Furthermore, HDM physically interacts with both MEI-9 and ERCC1 in a yeast two-hybrid assay. We conclude that HDM, MEI-9, MUS312, and ERCC1 form a complex that resolves meiotic recombination intermediates into crossovers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromosome Mapping
  • Crossing Over, Genetic* / drug effects
  • DNA Breaks, Double-Stranded / drug effects
  • DNA Repair* / drug effects
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / metabolism*
  • Drosophila Proteins / chemistry
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / cytology
  • Drosophila melanogaster / drug effects
  • Drosophila melanogaster / enzymology*
  • Drosophila melanogaster / genetics
  • Meiosis*
  • Methyl Methanesulfonate / toxicity
  • Molecular Sequence Data
  • Mutagens / toxicity
  • Mutation / genetics
  • Nondisjunction, Genetic / drug effects
  • Nuclear Proteins / metabolism*
  • Protein Binding / drug effects
  • Protein Subunits / metabolism
  • Two-Hybrid System Techniques

Substances

  • DNA-Binding Proteins
  • Drosophila Proteins
  • ERCC1 protein, Drosophila
  • MEI-9 protein, Drosophila
  • Mutagens
  • Nuclear Proteins
  • Protein Subunits
  • hdm protein, Drosophila
  • Methyl Methanesulfonate